Електронний репозитарій ПДАУ
Полтавський державний аграрний університет
Електронний репозитарій (сховище, архів) наукових публікацій – електронний архів результатів науково-дослідної роботи, публікацій науковців, викладачів, аспірантів та студентів університету, кваліфікаційних робіт студентів для їх централізованого зберігання та надання відкритого доступу до них світовій академічній спільноті у режимі онлайн.
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Case study: Influence of probiotics-based products on phytopathogenic bacteria and fungi in agrocenosis
(2026-03-02) Писаренко, Павло Вікторович; Pysarenko, P. V.; Samoilik M.; Тараненко, Анна Олексіївна; Taranenko, A. O.; Tsova, Yu.; Sereda, M.
In recent years bacterial diseases of agricultural plants have reached a new level of epiphytoty and they appear as an essential factor that influences crop yield. It is important to use products, which provide the eradication of pathogenic agents and the prevention of their development and propagation. The objective of our study is to define the effect of probiotics, based on Bacillus subtillis, on bactericidal and fungicidal activities, and to develop the recommendations for probiotics application in a biological system of plant protection. The results show that studied solutions Sviteco-PPW, Sviteco-OPL and Sviteco-Agrobiotic 01 affect phytopathogenic bacteria. Sviteco-Agrobiotic-01 either is the most active product, in its native state or diluted. It has demonstrated high antagonizing activity against all studied phytopathogenic bacteria. Hence, Sviteco-Agrobiotic-01 has the best potential to be used in the biological protection system of grain crops, grain legumes, vegetables from the most dangerous and widely spread pathogenic bacteria. Research results don't show a significant effect of studied probiotics on phytopathogenic fungicidal activity.
Efficiency of probiotic application for the remediation of contaminated soils in agrocenoses
(2026-03-02) Писаренко, Павло Вікторович; Pysarenko, P. V.; Samoilik, M.; Тараненко, Анна Олексіївна; Taranenko, A. O.; Mostoviak, I.; Лавріненко, Інна Вікторівна; Lavrinenko, I.; Shpyrna, V.
The problem of soil contamination is relevant today. Soil contamination is generally associated with intensive industrial activities, inadequate waste management, mining, military activities, or accidents. Pollutants (e.g. heavy metals) are accumulating in soil and have major indirect impacts on biodiversity, quality of groundwater resources, and food safety. Soil contamination of agricultural land is a particular threat. Due to the military action and other conditions, the numbers of sites where potentially polluting activities have taken place are increasing in Ukraine. The cultivation of agricultural crops on soils with a medium or high level of toxicity is only possible after the re mediation of this area. The use of biological methods to intensify soil remediation processes, thereby reducing the additional burden on the environment, is becoming more widespread. The application of probiotics is an innova tive and ecological method for the restoration of contaminated soils. This will enable to improve the conceptual approaches to the implementation of an ecologically safe model for the restoration of contaminated land under the conditions of military action in Ukraine. The aim of the research was to assess the phytotoxic effect of soil con taminated with heavy metals and petroleum products before and after probiotic application. The seedling method was used to determine the remediation potential of contaminated soils. An express test of Triticum aestivum was used to determine soil phytotoxicity. The research results show the negative impact of soil contamination with heavy metals and petroleum products on the biometric indices of Triticum aestivum plants and positive dynamics of biometric indices of Triticum aestivum in variants after probiotic treatment (86–92% compared to the control). The obtained data show that a significant phytotoxic effect is observed by all biometric indices of Triticum aesti vum in all studied variants. The highest phytotoxic effect (33.56–42.70%) was observed in variants with combined contamination (PP+Zn+Pb). The results of probiotic application show a phytotoxic effect of less than 20% by all biometric indices of Triticum aestivum for all studied variants. Therefore, the results of the research can be used to develop recommendations for the remediation of land contaminated by military actions in Ukraine and the creation of sustainable agroecosystems.
Effect of probiotic treatment on the microbiological activity of Ukrainian typical black soil
(2026-03-02) Писаренко, Павло Вікторович; Pysarenko, P. V.; Samoilik, M.; Тараненко, Анна Олексіївна; Taranenko, A. O.; Тараненко, Сергій Володимирович; Taranenko, S. V.; Bybyk, Y.
The search for novel substances that promote the establishment of a microbial community and facilitate optimal humification processes while increasing soil organic matter content offers an opportunity for land restoration. The objective of study was to determine the abundance of ecological and trophic groups of soil microorganisms and the intensity of microbiological processes when subjected to probiotic treatment at various concentrations and doses in typical black soil. The eco-trophic groups of soil microorganisms were identified by inoculating dilutions of soil suspensions onto selective nutrient media. The direction and intensity of soil microbiological processes were assessed using the mineralization-immobilization, oligotrophy, and pedotrophy indexes. The results demonstrate that probiotics has a positive impact on the microbiological activity of the soil, leading to an increase in the number of ecological and trophic groups of soil microorganisms during both spring and autumn seasons. Notably, a significant effect on the soil microorganism conditions was observed after 30 days of probiotic treatment. Probiotics exhibit a favorable influence on microbiological processes within the soil, fostering conducive conditions for the development of soil microorganisms and the formation of humus. The most effective concentration of probiotics for promoting the functionality of microbial communities in black soils is determined to be 10%, accompanied by a dose of 100 l ha-1. Consequently, the application of probiotic treatment at a concentration of 10% and a dose of 100 l/ha-1 holds the potential to enhance the biological state of the soil, restore soil microbial diversity, and serve as an environmentally safe fertilizer.
Ecotoxicological assessment of waste from oil production
(2026-03-02) Писаренко, Павло Вікторович; Pysarenko, P. V.; Samojlik, M.; Тараненко, Анна Олексіївна; Taranenko, A. O.; Лавріненко, Інна Вікторівна; Lavrinenko, I.; Mostoviak, I.
Utilization of mineralized fossil water (MFW), as a by-product of oil production is a special environmental prob lem in oil and gas areas. In Ukraine the annual production of mineralized fossil water together with oil is estimated at about 20 million tons. Taking into account that MFW is an aby-product of oil production, they are usually placed in waste disposal sites and can contaminate environment. It was determined that toxicological properties of min eralized fossil water have not been studied so far. There is the need for study of toxicological features of the MFW which will allow to determine its hazard class and to develop some measures for safe usage of this drug for making the agriculture more ecological. Thus, in the work we have made the assessment of ecotoxicological properties of MFW as an environmentally safe substitute for agrochemicals. It was established that MFW is referred to low-tox ic compounds. LD50 for oral introduction of the compound in the bodies of white female rats is more than 21,000 mg/kg, female mice – 31,000 mg/kg, male mice – more than 20,000 mg/kg; when applied to the skin of rats – more than 8000 mg/kg. The value of LD50 when applied to the inhalation routes of entry for female rats is more than 5000 mg/kg. It was justified that by oral route of entry and skin penetration the MFWs belong to the IV class of danger, and by inhalation route of entry – to III class. It was also determined that MFW does not have an irritating effect on the skin and mucous membranes of the eyes. There is also no sensitizing effect on the body of guinea pigs. Studies have shown that the cumulative properties of MFW are weakly represented. The accumulation coefficient is greater than 5. At a dose of 4260 mg/kg MFW has cardiotoxic effect, it affects the process of erythropoiesis and increases filtration and excretory function of the kidneys. Ineffective dose for rats in a subchronic experiment is 2130 mg/kg. These results of ecotoxicological study of MFW make it possible to confirm that its usage is safe in the agricultural system, especially as environmentally friendly organo-mineral fertilizer, herbicide, and fungicide.
Assessment of the soil microbial community under energy crops (Panicum virgatum L. and Miscanthus giganteus): a case study from Ukraine
(2026-03-02) Тараненко, Анна Олексіївна; Taranenko, A. O.; Тараненко, Сергій Володимирович; Taranenko, S. V.; Кулик, Максим Іванович; Kulyk, M. I.; Rytchenko, A.; Teteriuk, R.
Bioenergy crops are a promising alternative for energy production. They can be grown on inaccessible, degraded, marginal land that is not economically viable for traditional agriculture. At the same time, a broader evaluation of the environmental and ecological impact of energy crop cultivation on land is needed. There are still knowledge gaps regarding the mechanisms underlying soil carbon accumulation, especially concerning the involvement of the soil microbiome in facilitating these processes during energy crop cultivation. The present study determined the main ecotrophic and taxonomic groups of soil microbial communities and the direction of soil microbiological processes under Panicum virgatum L. and Miscanthus giganteus growth. Research results showed an initial impact of energy crop growth on soil microbial communities in the soil-climatic conditions of Ukrainian forest-steppe zones. Soils under energy crops are characterized by the highest abundances of the investigated ecological and trophic microbial groups (by 1.2-3 times) compared to control (soil under grassland). The coefficients of microbiological processes determine the high potential for increasing soil fertility under energy crop cultivation. Statistical analysis of the results confirmed a medium and strong correlation between soil microbiological parameters and soil organic carbon content during energy crop growth. Therefore, energy crops can act as ecosystem engineers, improving soil biological and chemical properties and supporting soil ecosystem sustainability.