Кафедра паразитології та ветеринарно-санітарної експертизи
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Item DESCRIPTION OF THE NEMATODE DIROFILARIA REPENS (NEMATODA, ONCHOCERCIDAE) PARASITIC IN DOGS IN UKRAINE(2023) Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Кравченко, Сергій Олександрович; Kravchenko, S. O.; Киричко, Олена Борисівна; Kyrychko, O. B.; Дмитренко, Надія Іванівна; Dmytrenko, N. I.; Сизоненко, Наталія Миколаївна; Syzonenko, N. M.; Опара, Надія Миколаївна; Opara, N. M.Dirofi lariasis is a zoonotic disease of domestic and wild carnivorous animals and humans. Subcutaneous migration of the parasite Dirofi laria repens Railliet & Henry, 1911 can lead to the local swellings with changeable localization. In humans, this parasite can cause the damage of lungs, male genitalia, and eyes. Th e range of D. repens is constantly spreading to new areas, and, considering the zoonotic potential of this parasite, it is important to widen the existing data concerning the morphology of D. repens. Present study provides the morphometric description of nematodes D. repens, obtained from subcutaneous tissue of dogs in Ukraine. Th e aim of this study was to make an accent on the key features for performing the identifi cation of parasites of this species. For males, 27 morphometric traits were identifi ed, characterising the total size of the body and the esophagus, the thickness of the cuticle in the region of the mouth opening, the localisation of the nerve ring and the cloaca, the morphological structure and metric parameters of the spicules, and the length of the tail. For females, 18 morphometric traits were identifi ed that, in addition to the common parameters, characterise cuticle thickness in the region of the vulva, localisation of the vulva and anus, body width in the regions of the anus and vulva, and tail length. Th is report widens the existing data on the morphological and metric characteristics of nematodes D. repensPublication In vitro tests of effect of disinfectants on the viability of Heterakis gallinarum nematode eggs during embryogenesis(2024) Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Омельченко, О. В.; Omelchenko O. V.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Дмитренко, Надія Іванівна; Dmytrenko, N. I.; Крикунова, Валентина Юхимівна; Krykunova, V. E.; Передера, Олена Олександрівна; Peredera, O. O.; Тагільцева, Яніна Михайлівна; Tahiltseva, Y. М.Affecting the transmission of an infection, objects of the external environment become contaminated with pathogens as a result of their massive release by the definitive host during exogenous stages of parasites’ development, which is an important chain of their epizootic process. Therefore, disinfestation plays an important role in the complex of measures for the prevention and control of poultry helminthiases, including Heterakis gallinarum infection of chickens. The purpose of the research was to investigate in laboratory conditions the ovicidal efficiency of modern disinfectants in relation to the viability of developing eggs of H. gallinarum isolated from infested chickens. "Hermecid-VS" (didecyldimethylammonium chloride, glutaraldehyde, benzalkonium chloride) and "Arquadez-plus" (dimethyldialkylammonium chloride, didecyldimethylammonium chloride, tetrasodium salt) disinfectants were tested. These preparations had a high level of ovicidal efficiency against the eggs of Heterakis, "Hermecid-VS" in 0.25% and 0.5% concentration for exposures of 10–60min (93.5–100.0%), "Arquadez-plus" in 1.0% concentration for exposure of 60 min (93.1%), and at 1.5% (96.8–100.0%) and 2.0% (100.0%) concentrations regardless of exposure. The ovicidal effect of disinfectants was characterized by morphological changes in the nematode eggs when using "Hermecid-VS" – accumulation of air bubbles under the shell, thinning and deformation of the shell, death and gradual resorption of the embryo, loosened egg shell and terminated embryonic development, deformation and thinning of egg the shell, and death of the embryo at the stage of formation of the larva; under the effect of "Arquadez-plus", a stop in development at the stage of cleaving blastomeres, destruction of the shell, death and evacuation of the embryo from the egg, shrinkage and gradual defragmentation of the embryo, death of the larva and its defragmentation. Under the action of disinfectants, the metric parameters of Heterakis eggs also changed. Under the influence of "Hermecid-VS" at all concentrations, the length of the eggs and the thickness of the shell were larger (by 2.9–5.4% and 26.7–46.7%), and their width was smaller (by 5.5–6.9%). With the effect of "Arquadez-plus", the length of the eggs was greater (by 0.4–2.0%), and the width was smaller (by 0.7–4.9%) only when using 0.1–2.0% concentrations of the agent. Heterakis egg shell thickness increased under the influence of "Arquadez-plus" in concentrations of 0.25–2.0% (by 33.3–40.0%). The conducted studies allow us to recommend "Hermecid-VS" and "Arquadez-plus" disinfectants in specified concentrations and modes of their use for maintaining epizootic well-being in the complex of measures against Heterakisinfection of chickesnItem Morphological Characteristics of Parasitic Nematodes Trichuris sylvilagi (Nematoda, Trichuridae)(2022-06) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Стибель, В.; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Нагорна, Л.; Dmytrenko, N. I.; Дмитренко, Надія Іванівна ; Titarenko, O. V.; Тітаренко, Олена Вікторівна ; Дубова, О.; Makarets, S.; Макарець, Світлана Володимирівна ; Filonenko, S. V.; Філоненко, Сергій Васильович; Pishchalenko, M. A.; Піщаленко, Марина Анатоліївна; Kone, M. S.; Коне, Мохамед Сумана ; Ilchenko, A.; Ільченко, Алла Михайлівна