Кафедра інфекційної патології, гігієни, санітарії та біобезпеки

Постійне посилання на фонд

https://dspace.pdau.edu.ua/handle/123456789/5082

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Перегляд Кафедра інфекційної патології, гігієни, санітарії та біобезпеки за Ключові слова "chicken"

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  • Ескіз
    Публікація
    In vitro tests of effect of disinfectants on the viability of Heterakis gallinarum nematode eggs during embryogenesis
    (2024) Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Омельченко, Ольга Вікторівна; Omelchenko O. V.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Дмитренко, Надія Іванівна; Dmytrenko, N. I.; Крикунова, Валентина Юхимівна; Krykunova, V. E.; Передера, Олена Олександрівна; Peredera, O. O.; Тагільцева, Яніна Михайлівна; Tahiltseva, Y. М.
    Affecting the transmission of an infection, objects of the external environment become contaminated with pathogens as a result of their massive release by the definitive host during exogenous stages of parasites’ development, which is an important chain of their epizootic process. Therefore, disinfestation plays an important role in the complex of measures for the prevention and control of poultry helminthiases, including Heterakis gallinarum infection of chickens. The purpose of the research was to investigate in laboratory conditions the ovicidal efficiency of modern disinfectants in relation to the viability of developing eggs of H. gallinarum isolated from infested chickens. "Hermecid-VS" (didecyldimethylammonium chloride, glutaraldehyde, benzalkonium chloride) and "Arquadez-plus" (dimethyldialkylammonium chloride, didecyldimethylammonium chloride, tetrasodium salt) disinfectants were tested. These preparations had a high level of ovicidal efficiency against the eggs of Heterakis, "Hermecid-VS" in 0.25% and 0.5% concentration for exposures of 10–60min (93.5–100.0%), "Arquadez-plus" in 1.0% concentration for exposure of 60 min (93.1%), and at 1.5% (96.8–100.0%) and 2.0% (100.0%) concentrations regardless of exposure. The ovicidal effect of disinfectants was characterized by morphological changes in the nematode eggs when using "Hermecid-VS" – accumulation of air bubbles under the shell, thinning and deformation of the shell, death and gradual resorption of the embryo, loosened egg shell and terminated embryonic development, deformation and thinning of egg the shell, and death of the embryo at the stage of formation of the larva; under the effect of "Arquadez-plus", a stop in development at the stage of cleaving blastomeres, destruction of the shell, death and evacuation of the embryo from the egg, shrinkage and gradual defragmentation of the embryo, death of the larva and its defragmentation. Under the action of disinfectants, the metric parameters of Heterakis eggs also changed. Under the influence of "Hermecid-VS" at all concentrations, the length of the eggs and the thickness of the shell were larger (by 2.9–5.4% and 26.7–46.7%), and their width was smaller (by 5.5–6.9%). With the effect of "Arquadez-plus", the length of the eggs was greater (by 0.4–2.0%), and the width was smaller (by 0.7–4.9%) only when using 0.1–2.0% concentrations of the agent. Heterakis egg shell thickness increased under the influence of "Arquadez-plus" in concentrations of 0.25–2.0% (by 33.3–40.0%). The conducted studies allow us to recommend "Hermecid-VS" and "Arquadez-plus" disinfectants in specified concentrations and modes of their use for maintaining epizootic well-being in the complex of measures against Heterakisinfection of chickesn
  • Ескіз
    Публікація
    Inhibitory properties of disinfectants on the sporogony of Eimeria tenella (Protista, Eimeriidae)
    (2025) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Melnychuk, V. V.; Мельничук, Віталій Васильович; Hodyna, V.; Mykhailiutenko, S. M.; Михайлютенко, Світлана Миколаївна; Kruchynenko, O. V.; Кручиненко, Олег Вікторович; Omelchenko, A.; Avramenko, N. О.; Авраменко, Наталія Олексіївна; Mazannyi, O.
    he protozoa of kingdom Protista are widespread pathogens of gastrointestinal protozoal diseases in chickens and cause significant economic losses to farms. Prevention includes a set of measures to treat poultry with effective drugs, and disinfect the environmental objects using modern, safe and beneficial means. For this purpose, it is recommended to use disinfectants that have been tested for their disinfestation effect. The aim of the research was to investigate the disinfestation effect of chemicals on the process of sporulation of Eimeria tenella oocysts isolated from chickens in laboratory conditions. It was found that the disinfectants "Virosan" (active substances – alkyldimethylbenzylammonium chloride and glutaraldehyde) and "Yoderin" (contains iodophores) had disinvasive properties, but their indicators of inhibitory action against sporogony of E. tenella oocysts were not the same. The most effective was the agent alkyldimethylbenzylammonium chloride and glutaraldehyde mixture, with a 100% inhibitory effect when used at a 0.5% concentration and exposure of 150 minutes. At lower exposures and concentrations, its indicators of inhibitory action ranged within the following limits: at 0.1% concentration to 57.0%, at 0.25% concentration to 84.5%. The inhibitory effect of the iodophor-based disinfectant was lower and varied depending on the exposure (from 30 to 150 min) within the following limits: at 0.5% concentration to 35.7%, at 1.0% concentration to 68.2%, at 2.0% concentration to 93.1%. In addition to stopping the development of Eimeria oocysts, the disinfectants induced changes in their morphological and metric indicators. Metric indicators of oocysts under the action of disinfectants were characterized by lower values of their width (by 4.8–8.7%), and under the action of the iodophor-based agent, by a decrease in the ratio of length / width of oocysts (by 11.0%). The obtained results of the inhibitory action of the disinfectants present prove the possibility of using these agents to combat and prevent eimeriasis in chickens