Факультет ветеринарної медицини

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    Ефективність методів лабораторної діагностики асоціативної трихурозно-токсокарозної інвазії у собак
    (2025) Yevstafieva, V. O.; Kryvoruchenko, D. O.; Євстаф’єва, Валентина Олександрівна; Криворученко, Д. О.
    Gastrointestinal nematodoses are widespread and dangerous parasitic diseases of dogs, and it is known that some of them have zoonotic potential and pose a threat to human health. For rapid intravital diagnosis of gastrointestinal nematodoses in dogs, a wide range of salts and other substances is available for use in flotation examinations. Since polyparasitism is a very common phenomenon, there is a need for sensitive and accurate diagnostic tools that are easy to apply and allow simultaneous detection of several pathogens during integrated control of parasitic mixed infections. The aim of this study was to determine the sensitivity of known coproovoscopic methods in dogs with associative trichurosis–toxocarosis infection. The research was carried out at the private veterinary clinic “Dovira” (Kharkiv) and at the Parasitology Laboratory of Poltava State Agrarian University (Poltava). The following flotation methods were tested: Kotelnikov–Khrenov (using an ammonium nitrate solution), Sorokova (using a combined solution of calcium and ammonium nitrate), Petrenko (using a combined solution of calcium nitrate, sugar, and sodium chloride), and Melnychuk (using a combined solution of calcium nitrate and sodium chloride). The conducted studies established that in dogs with trichurosis–toxocarosis infection, all tested methods, regardless of the exposure time of fecal samples, allowed simultaneous detection of Toxocara and Trichuris eggs, although the intensity of infection varied. The highest intensity values of toxocarosis and trichurosis infection were detected when applying the Melnychuk method with a fecal sample exposure of 15 min, reaching 69.3 and 84.4 eggs/g, respectively. This method demonstrated higher sensitivity in dogs with trichurosis–toxocarosis infection compared with the Kotelnikov–Khrenov method by 21.9–73.1 %, the Sorokova method by 21.2– 30.9 %, and the Petrenko method by 27.6 %. The obtained results allow recommending the Melnychuk method, which uses a combined solution of calcium nitrate and sodium chloride as the flotation medium, for effective intravital laboratory diagnosis of the associative course of trichurosis and toxocarosis in dogs