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https://dspace.pdau.edu.ua/handle/123456789/21

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Зараз показуємо 1 - 20 з 1582

30 років від часу розробки першої ДНК- вакцини.
(2022-11) Тітаренко, Олена Вікторівна; Микитенко, А. О.
A clinical case of a mixed testicular tumour with cryptorchidism in a dog of the Yorkshire Terrier breed
(2023) Канівець, Наталія Сергіївна; Коломак, Ігор Олегович; Кравченко, Сергій Олександрович; Дмитренко, Надія Іванівна; Бурда, Тетяна Леонідівна; Kanivets, N. S.; Kolomak, I. O.; Kravchenko, S. O.; Dmytrenko, N. I.; Burda, T. L.
Histological examination of testicular tumours in cryptorchid males allows determining the nature of neoplasia, prognosis of long-term consequences in the form of metastases, and defining the optimal method of treatment of the animal, which determines the relevance of research in this area. The aim of the work is to determine the pathohistological structure of neoplasia of the left, cryptorchid, and morphology of the contralateral testes in a Yorkshire Terrier male with unilateral cryptorchidism. General clinical, ultrasonographic, radiological and histological methods were used in the examination of the animal. The article presents data on a mixed tumour of the intraperitoneal left testis of a 6-year-old Yorkshire Terrier, which is an atypical clinical case. It was found that the characteristic ultrasonographic features of the cryptorchid left testis tumour were numerous isoechoic foci of different diameters (from 4 to 26 mm), hyperechoic septa, and tuberous contours with a hyperechoic capsule. At laparotomy, the altered testis had an uneven surface saturated with blood vessels. The section showed milky-white neoplastic areas separated by fibrous bands that did not correspond to the normal structure of the organ. Histological examination of the parenchyma revealed areas of similar rounded cells in the form of a group of shapeless masses separated by a fibrous barrier. Neoplastic tubules had a developed fibrovascular stroma with an eosinophilic necrotic area. The protein membrane also had numerous fibrous formations. The layers of multifaceted cells were located perpendicular to the basal lamina, often with central necrosis, and the testicular mass contained multifocal haemorrhages. Such signs are characteristic of the lesion of Sertoli cells and interstitial Leydig cells, which indicated a mixed type of tumour of the left extraperitoneal testis of the Yorkshire Terrier. The contralateral right testis was unchanged and corresponded to the natural morphological and histological structure. The prostate was normal on macroscopic and histological examination, which has not been previously reported. Six-month follow-up of the dog showed no signs of metastatic process. The practical significance of the work is to obtain new scientific knowledge about the development of mixed tumours of the testis in cryptorchid males and the prospects for the treatment of diseased animals
A panel of five microsatellites as an alternative to mitogenome sequencing for investigating the genetic diversity of Echinococcus granulosus sensu stricto from global to farm scale
(2026) Umhang, G.; Bastid, V.; Rinaldi, L.; Pepe, P.; Ciccone, E.; Sotiraki, S.; Ligda, P.; Oudni-M’rad, M.; M’rad, S.; Lahmar, S.; Said, Y.; Elmehatli, K.; Ahmed, H.; Kuzmina, T.; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Aghayan, S. A.; Gevorgyan, H.; Simsek, S.; Ponce-Gordo, F.; Benchikh El Fegoun, M. C.; Berbri, I. E.; Fihri, O. F.; Saarma, U.; Grenouillet, F.; Bou´, F.; Aguayo, J.
While the molecular identification of Echinococcus granulosus species is essential for surveillance, a detailed investigation of the parasite’s genetic diversity would offer a more accurate understanding of its transmission. This may be done by partial or whole mitochondrial genome sequencing, but both cost and time requirements limit routine application. Microsatellites are an alternative approach as they are a rapid, simple, highly discriminative, and low-cost tool. Our research project was designed to obtain a panel of microsatellite targets for population genetic analyses to describe the genetic diversity of E. granulosus sensu stricto (s.s.) at the global to farm scale. A panel of five informative microsatellite loci for population genetic analysis of E. granulosus s.s. was developed and validated. A total of 145 isolates from 11 countries were used to evaluate the panel at the international level. Additionally, 131 samples from sheep and cattle across six regions in southern Italy were analyzed to assess the panel’s usefulness at both the regional and farm scale. Population genetics showed high genotypic diversity. Simpson’s index gave high values (0.94–0.98) for the expected heterozygosity (0.80 to 0.87). Discriminant analysis of principal components (DAPC) revealed the structure of populations for each country, with Greece distinctly distant from Algeria, Italy, and Tunisia. This was also confirmed by FST values. DAPC further revealed a genetic structure among the farms from the Italian regional sample set, with three different clusters explained by different farming practices. This microsatellite panel can provide data relevant for E. granulosus s.s. population genetics from global to farm scale, making it a low-cost, simple alternative to full mitochondrial genome sequencing for analyzing numerous samples and obtaining a detailed overview of genetic diversity, an input that is useful for cystic echinococcosis control programs
Activity indicators of some enzymes in the blood serum of dogs at different intensities of dipylidium infection
(2026) Nikitan, А. D.,; Melnychuk, V. V.; Мельничук, Віталій Васильович
The article presents the findings of a study on the activity of key blood serum enzymes in dogs with varying infection intensities of Dipylidium caninum, conducted between 2024 and 2026 in Poltava. Examining four groups of young dogs (under 1 year of age), the study demonstrated that D. caninum infection induces clear dose-dependent toxic effects on the host organism. Even at low infection intensity (up to 2 cocoons/g of feces), statistically significant increases were observed in ALT (by 21.3%), AST (by 12.4%), and alkaline phosphatase (by 12.0%) compared to healthy control dogs. With high infection intensity (8–14 cocoons/g), enzyme elevations were most pronounced: ALT increased by 34.5%, AST by 26.9%, alkaline phosphatase by 33.0%, along with a significant 19.8% increase in GGT activity. These biochemical alterations reflect increasing hepatobiliary stress and metabolic dysfunction as parasitic burden rises.
An Investigation of Embryo and Eggshell Development in Trichuris suis (Nematoda, Trichuridae) under Laboratory Conditions
(2016-06-15) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Юськів, І. Д.; Melnychuk, V. V.; Мельничук, Віталій Васильович
Analysis of the treatment regimen efficacy for columnaris disease in Pterophyllum scalare
(2020-08) Lavrinenko, I. V.; Shulga, L. V.; Peredera, O. O.; Zhernosik, I. A.; Лавріненко, Інна Вікторівна; Передера, Олена Олександрівна
Analysis of ultrastructural morphometric changes of pigeon kidneys affected by colibacteriosis
(2020-01) Berdnyk, V. P.; Nedosekov, V. V.; Kolomak, I. O.; Kyrychko, O. B.; Киричко, Олена Борисівна; Коломак, Ігор Олегович
Biological specifics of exogenous development of Oxyuris equi nematodes (Nematoda, Oxyuridae)
(2020) Yevstafieva, V. O. ; Євстаф’єва, Валентина Олександрівна; Prykhodko, Y. О.; Kruchynenko, O. V. ; Кручиненко, Олег Вікторович; Mykhailiutenko, S. M.; Михайлютенко, Світлана Миколаївна; Kone, M. S. ; Коне, Мохамед Сумана; Кручиненко, Олег Вікторович
Bird Lice (Mallophaga, Philopteridae, Menoponidae) of Domestic Pigeons on Specialized Pigeon Breeding Farms in Poltava
(2017) Kolomak, I. O.; Kruchynenko, O. V.; Кручиненко, Олег Вікторович; Коломак, Ігор Олегович
Bird Lice (Mallophaga, Philopteridae, Menoponidae) of Domestic Pigeons on Specialized Pigeon Breeding Farms in Poltava
(2017-12) Kolomak, I. O.; Kruchynenko, O. V.; Коломак, Ігор Олегович; Кручиненко, Олег Вікторович
Body weight of different genealogical lines of the gray Ukrainian breed of heifers as a factor of determining its consolidation and crossing efficiency
(2021) Voitenko, S. L.; Dzhus, P. P.; Sidorenko, E. V.; Петренко, Максим Олександрович
Chewing Lice (Order Mallophaga, Suborders Amblycera and Ischnocera) Fauna of Domestic Chicken (Gallus gallus domesticus) in Ukraine
(2015-11-26) Євстаф’єва, Валентина Олександрівна ; Yevstafieva, V. O.
Chlamydial infection monitoring within wild mammals in Ukraine
(2019) Коне, Мохамед Сумана
CHLAMYDIAL INFECTION MONITORING WITHIN WILD MAMMALS IN UKRAINE
(2019) Shcherbakova, N. S.; Zezekalo, V.K; Peredera, S. B.; Peredera, Zh.O; Kone, M. S.; Kravchenko, S. O.; Kanivets, N. S.; Щербакова, Наталія Сергіївна; Канівець, Наталія Сергіївна; Кравченко, Сергій Олександрович; Коне, Мохамед Сумана; Передера, Сергій Борисович
Clinical and morphological justification of the treatment of purulonecrotic lesion in deep structures of limb distal segment in pig: case report
(2020-12) Kyrychko, B. P.; Zvenigorodska, T. V.; Kravchenko, S. O.; Kyrychko, O. B.; Semirenko, Vitalii; Киричко, Борис Павлович; Звенігородська, Таміла Владиславівна; Кравченко, Сергій Олександрович; Киричко, Олена Борисівна
Clinical and morphological manifestation of the visceral form of candidiasis in the domestic dog (Canis familiaris)
(2024) Skrypka, M.; Kolych, N.; Дмитренко, Надія Іванівна; Dmytrenko, N. I.
The relevance of this research lies in the current lack of information regarding the pathological manifestations of fungal infections in the parenchymal organs of animals, the manifestations and the host's response to the fungus. Diagnosis of visceral mycoses is quite complex and insufficiently substantiated. A significant factor is the absence of clear criteria for pathological changes in visceral mycoses and differential diagnosis from similar diseases. This study aimed to reveal and establish the features of the clinical and morphological manifestation of the visceral form of candidiasis in the domestic dog (Canis familiaris). The study employed the following main methods: morphologic and biochemical blood analysis, post-mortem examination, and cytological and histological studies. When conducting biochemical analyses of blood serum in dogs, it was found that the glucose concentration was 2.17 mmol/L, which is almost three times lower than the lower limit of the norm, while the creatinine content exceeded the norm by four times and corresponded to a value of 560.4 mmol/L. As a result of serum analysis, an increase in the content of total and direct bilirubin was also recorded. In particular, the content of total bilirubin exceeded the physiological limit by 20 times and amounted to 222.68 µmol/L, while the level of direct bilirubin increased almost 10-fold and corresponded to a value of 59.4 µmol/L. The activity of aminotransferases, gamma-glutamyl transpeptidase, amylase, and alkaline phosphatase in the blood serum of sick animals increased significantly. Key pathological features of visceral mycoses in domestic dogs include haemolytic jaundice with extensive haemorrhages in organs and tissues. Hepatitis and nephritis, diagnosed in sick dogs, are the result of a generalised infectious process, the etiological factor of which is visceral candidiasis. The obtained data are of practical value for practising veterinarians in the issue of differential diagnosis of candidiasis, revealing the features of clinical and morphologic changes in the visceral form of mycoses in dogs
Comparative effectiveness of disinfectants used in vitro during embryogenesis of Toxocara cati nematodes
(2026) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Perviy, A.; Melnychuk, V. V.; Мельничук, Віталій Васильович; Petrenko, M. O.; Петренко, Максим Олександрович; Kone, M. S.; Коне, Мохамед Сумана; Pishchalenko, M. A.; Піщаленко, Марина Анатоліївна; Dedukhno, A. V.; Дедухно, Алла Володимирівна; Krasota, O. G.; Красота, Олена Григорівна
Toxocara cati helminths are widespread zoonotic pathogens that cause diseases in various wild and domestic animals, as well as humans, both definitive and paratenic hosts. Mature toxocaras release a significant number of eggs with the host’s feces, which pollute the external environment and are also resistant to adverse factors. To efficiently combat and prevent anthropozoonotic toxocariasis, it is necessary to carry out disinfestation using relevant agents. The aim of our research was to determine in laboratory conditions the ovicidal efficacy of disinfectants against embryogenesis of T. cati nematodes isolated from cats. It was found that the tested agents had different ovicidal efficacy on the test culture of T. cati eggs, depending on the composition of the disinfectant, its concentration and exposure. The most effective was "Iodine monochloride" (active ingredients include iodine monochloride and hydrochloric acid) at a concentration of 3.0%, which showed a high level of disinvasive action (96.7%) only after 60 min of exposure. The disinfectants "Dezirex Forte" (glutaraldehyde, benzalkonium chloride, didecyldimethylammonium chloride, N,N-didecyl-N-methyl- oly(oxyethyl)ammonium propionate, isopropanol), "Parvo-Virucide" (glutaraldehyde, benzyldimethyltetradecylammonium chloride) and "Multiclean Aqua" (benzalkonium chloride, didecyldimethylammonium chloride, glutaraldehyde, isopropanol, polyhexamethylene biguanide hydrochloride) showed satisfactory levels of ovicidal efficacy against T. cati eggs. The maximum values of the harmful effect were established for 2.0% "Dezirex Forte" at exposures of 30 and 60 minutes, 71.4 and 79.3%, respectively; 2.0% "Parvo-Virucide" at exposures of 30 and 60 minutes – 74.5 and 81.5%, respectively; 1.0% "Multiclean Aqua" at exposure of 60 minutes – 61.4%. Thus, we recommend the disinfectant "Iodine monochloride" under a certain regime for the effective neutralization of T. cati eggs and for the prevention of environmental contamination.
Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)
(2018-11-14) Melnychuk, V. V.; Мельничук, Віталій Васильович ; Березовський, А. В.
Comparative morphology and biology of nematodes of genus Heterakis (Nematoda, Heterakidae), parasites of the domestic goose (Anser anser) in Ukraine
(2018-04-28) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Нікіфорова, О. В.; Супруненко, Костянтин Володимирович; Корчан, Леонід Миколайович; Локес-Крпука, Т.П.; Негребецький, Ігор Станіславович; Корчан, Микола Іванович; Suprunenko, K. V.
Comparison of antimicrobial activity of plant tinctures and chemical disinfectants
(2025) Kruchynenko, O. V.; Кручиненко, Олег Вікторович; Zamaziy, A. A.; Замазій, Андрій Анатолійович; Petrenko, M. O.; Петренко, Максим Олександрович; Lavrinenko, I. V.; Лавріненко, Інна Вікторівна; Khyl, A.
Disinfection in veterinary medicine is a complex of measures aimed at destroying pathogens of infectious animal diseases to ensure safe conditions for their keeping and prevent the occurrence and spread of infections. The pharmacological properties of plant extracts are of considerable significance for further research of substances as potential components of preventive veterinary medicinal products. The results of a study on the effectiveness of the following disinfectants are presented in the article: Iodoclean, Virosan, Ioderin, Multiclean Aqua, as well as chamomile and hawthorn tinctures. The antibacterial activity of the alcoholic tinctures and chemical disinfectants was assessed by measuring the zones of micro-organisms’ growth inhibition around the wells containing the test substances, using the agar well diffusion method. The study was aimed at comparing the effectiveness of 40 % chamomile tincture, 70 % hawthorn tincture, and Iodoclean, Ioderin, Multiclean Aqua, and Virosan chemical preparations against the sanitary indicator micro-organisms of Staphylococcus aureus, strain 209 and Escherichia coli, strain 1257.When determining the zone of inhibition of E. coli growth, Iodoclean demonstrated the highest activity with the zone of inhibition of 25 mm. Virosan was somewhat less active (24 mm), which is 1.04 times lower than Iodoclean. Multiclean Aqua and Ioderin disinfectants demonstrated the activity 1.19-1.25 times lower than Iodoclean. Chamomile tincture demonstrated the activity 2.08 times lower, and hawthorn tincture – 3.57 times lower, with the zone of inhibition of only 7 mm. In the study of the inhibition zones against Staphylococcus aureus, Virosan showed the greatest activity with the inhibition zone of 25 mm. Iodoclin and Multiclean Aqua were slightly less active, being 1.08 times lower than Virosan. Ioderin provided the inhibition zone of 22 mm, which is 1.13 times lower than that of Virosan. Chamomile and hawthorn tinctures turned out to be less effective compared to the other disinfectants. Their inhibition zones made 14 and 8 mm, respectively, which are 1.78 and 3.12 times lower than Virosan’s indicators

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