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Histological changes in the experimentally damaged bone tissue of rabbits following injection of stem cells

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dc.contributor.authorSavchuk, T. L.
dc.contributor.authorMazurkevich, A. Y.
dc.contributor.authorMalyuk, M. О.
dc.contributor.authorKharkevych, I. O.
dc.contributor.authorBokotko, R. R.
dc.contributor.authorKladnytska, L. V.
dc.contributor.authorMasalovych, Y. S.
dc.contributor.authorParamonova, Y. V.
dc.contributor.authorZhuk, Y. V.
dc.contributor.authorDymko, R. О.
dc.contributor.authorKruchynenko, O. V.
dc.contributor.authorКручиненко, Олег Вікторович
dc.date.issued2025
dc.description.abstractBone defects are the results of pathological factors that disrupt the integrity of the bones and cause losses of the bone tissue or its absence. Disruption or hindering of the regeneration processes of the damaged bone tissue due to complications occur at a quite high rate and are the main problems in the bone tissue engineering. An effective treatment option is mesenchymal stem cells of mammals. In fact, they are considered the most promising type of autoimmune and allogeneic material in the cell regeneration therapy. We conducted a histological analysis of the damaged tibia of the rabbits following the injection of allogeneic mesenchymal stem cells. For this purpose, we used the mesenchymal stem cells from the bone marrow of the rabbits. The cells were cultivated in a CO2 incubator using standard methods. The injury of the bone tissue was modeled using a surgical drill on the three-month-old rabbits of the chinchilla breed, in the middle third part of the diaphysis of the tibia. The animals were locally injected with allogeneic mesenchymal stem cells. The tissue samples from the defect region for histological studies were collected on days 3, 7, 14, 21, 28, and 42. The obtained histological sections from the injured area had been stained with hematoxylin-eosin and were analyzed under a microscope. The histological analysis of the experimentally damaged tibia revealed that the injection of allogeneic mesenchymal stem cells expedited the formation of fibrous connective tissue and fibrocartilage callus, stimulated osteogenesis, and promoted a consolidation of the bone tissue. At the same time, we observed the healing of the defect, which completed almost on day 28 of the study in the experimental animals, in contrast to day 42 in the control animals. We assume that mesenchymal stem cells – as multipotent stem cells – have immunomodulating properties and the capacity to osteogenically differentiate. Also, we think that allogeneic mesenchymal stem cells intensified the regeneration processes and enhanced the phases of reparative osteogenesis in the defect zone of the tibia
dc.identifier.citationSavchuk T. L., Mazurkevich A. Y., Malyuk M. О., Kharkevyc, I. O., Bokotko R. R., Kladnytska L. V., Masalovych Y. S., Paramonova Y. V., Zhuk Y. V., Dymko R. О., Kruchynenko O. V. Histological changes in the experimentally damaged bone tissue of rabbits following injection of stem cells. Regulatory Mechanisms in Biosystems. 2025. Vol. 16, No. 2. P. 1-9. DOI: https://doi.org/10.15421/0225091
dc.identifier.urihttps://dspace.pdau.edu.ua/handle/123456789/20821
dc.language.isouk
dc.subjectreparative osteogenesis
dc.subjectfibrocartilage callus
dc.subjectbone tissue
dc.subjectbone marrow
dc.subjecthistological section
dc.subjectosteocyte
dc.subjectosteoblast
dc.subjectfibroblast
dc.titleHistological changes in the experimentally damaged bone tissue of rabbits following injection of stem cells
dc.typeArticle
dspace.entity.typePublication
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Друковані видання. Кафедра інфекційної патології, гігієни, санітарії та біобезпеки