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Immunohistochemical characteristics of the rat ileum under impaired antioxidant defence induced by the administration of a complex of chemical food additives

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dc.contributor.authorBilash, S. M.
dc.contributor.authorOliinichenko, Ya. O
dc.contributor.authorPronina, O. M.
dc.contributor.authorShostya, A. M.
dc.contributor.authorShostia, A. M.
dc.contributor.authorШостя, Анатолій Михайлович
dc.contributor.authorKoptev, M. M.
dc.contributor.authorPirog-Zakaznikova, A. V.
dc.contributor.authorDonchenko, S. V.
dc.contributor.authorOleksiienko, V. V.
dc.contributor.authorMamai, O. V.
dc.contributor.authorOliinichenko, M. O.
dc.contributor.authorKoval, Yu. Р.
dc.contributor.authorKopytko, N. S.
dc.date.issued2026-01-13
dc.descriptionBilash S. M., Oliinichenko Y. O., Pronina O. M., Shostya A. M., Koptev M. M., Pirog-Zakaznikova A. V., Donchenko S. V., Oleksiienko V. V., Mamai O. V., Oliinichenko M. O., KovalY Р., Kopytko N. S. Immunohistochemical characteristics of the rat ileum under impaired antioxidant defence induced by the administration of a complex of chemical food additives. Reports of Morphology. 2025. Vol. 31 (4). P. 12-21. DOI: https://doi.org/10.31393/morphology-journal-2025-31(4)-02
dc.description.abstractThe use of chemical food additives can cause morphofunctional changes in various internal organs. This is accompanied by disruption of both local immune and antioxidant defences. Therefore, the aim of the study was to evaluate immunohistochemical changes in the ileum of rats under conditions of impaired antioxidant defence caused by the combined administration of monosodium glutamate, sodium nitrite and Ponceau 4R dye. The study was conducted on 70 sexually mature white rats, which were divided into a control group and six experimental groups of 10 animals each. The control group received saline, while the experimental groups were administered a solution of chemical food additives for 1, 4, 8, 12, 16 and 20 weeks. At the end of each experimental period, ileal tissue was collected. Immunohistochemical studies were performed on paraffin-embedded sections. CD4, CD8, CD20 and CD68 markers were used to assess the cellular and humoral components of immunity. The state of the antioxidant system was evaluated by determining the levels of superoxide dismutase and catalase activity. Statistical analysis of the results was carried out using the “InStat” software package. In the early stages of the experiment, an increase in the number of CD68⁺ and CD20⁺ cells was observed, reflecting an acute tissue response to the toxic effects of the food additive complex. From weeks 4-8 onwards, a gradual decrease in the expression of CD4⁺, CD8⁺, CD20⁺ and CD68⁺ was noted, indicating the depletion of the immunoregulatory and effector components of local immunity. The lowest level of all studied cell populations was recorded at week 16. At the same time, suppression of superoxide dismutase and catalase activity was detected. Their reduction in the early stages indicates activation of lipid peroxidation processes and accumulation of reactive oxygen species, which may contribute to structural damage to the cellular components of the intestinal wall. Despite activation of the antioxidant defence system at the intermediate stages, the subsequent decline in enzyme activity after 12 weeks suggests depletion of defence mechanisms and the development of oxidative stress. Partial restoration of enzymatic activity at the end of the experiment is likely to reflect compensatory tissue responses to prolonged exposure to the damaging factor. Thus, the combined administration of monosodium glutamate, sodium nitrite and Ponceau 4R dye induces significant morphofunctional changes in the rat ileal mucosa, characterised by disruption of the cellular composition of the immune system and decreased antioxidant defence activity.
dc.identifier.urihttps://dspace.pdau.edu.ua/handle/123456789/19988
dc.language.isoen
dc.subjectrats
dc.subjectdigestive tract
dc.subjectsmall intestine
dc.subjectileum
dc.subjectfood additives
dc.subjectmicroscopic changes
dc.subjectimmunohistochemical study
dc.subjectCD4
dc.subjectCD8
dc.subjectCD20
dc.subjectCD68
dc.subjectsuperoxide dismutase
dc.subjectcatalase
dc.subjectoxidative stress
dc.subjectmonosodium glutamate
dc.subjectsodium nitrite
dc.subjectPonceau 4R
dc.titleImmunohistochemical characteristics of the rat ileum under impaired antioxidant defence induced by the administration of a complex of chemical food additives
dc.typeArticle
dspace.entity.typePublication
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