Фахові видання
Permanent URI for this collection
Browse
Browsing Фахові видання by Author "Kolupaev, Yu. E."
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Effect of melatonin and hydropriming on germination of aged triticale and rye seeds(2024) Kolupaev, Yu. E.; Taraban, D. A.; Kokorev, A. I.; Yastreb, T. O.; Pysarenko, V. M.; Sherstiuk, E. L.; Karpets, Yu. V.; Писаренко, Віктор Микитович; Шерстюк, Олена ЛеонідівнаThe formation of reactive oxygen species, which initiate lipid peroxidation of membranes and disruption of all membrane-associated processes, is one of the critical factors causing seed senescence and loss of germinability. Melatonin is currently considered one of the powerful antioxidant bioregulators that influence many plant functions, including their resistance to stress factors and seed germination. However, the effect of melatonin on the germination of old triticale and rye seeds has not been specifically investigated. The work aimed to study the effect of melatonin treatment and the well-known procedure of hydropriming (soaking in water with subsequent drying of seeds) on the germination of seeds of triticale ‘Raritet’ and rye ‘Pamyat Khudoerka’ with low germination capacity and the state of antioxidant system in seedlings. Hydropriming had a small but significant effect on the germination of triticale seeds at p ≤ 0.05 and significantly increased the germination of rye seeds. Melatonin treatment positively impacted both crop seed germination and significantly exceeded the effects of hydropriming. The most significant effect was observed when melatonin was used at a concentration of 20 μM. The accumulation of shoot and root biomass also increased under hydropriming and (to a greater extent) melatonin. Melatonin treatment led to a significant decrease in the generation of superoxide anion radical and the content of hydrogen peroxide and malonic dialdehyde, a product of lipid peroxidation, in seedlings of both crops. Additionally, melatonin increased the activity of superoxide dismutase and catalase in both crops. The anthocyanin content increased in the seedlings of both cereals under the influence of melatonin. The effect of hydropriming on indicators characterising the prooxidant and antioxidant balance was insignificant. A conclusion was drawn on the ability of exogenous melatonin to enhance the germination of old triticale and rye seeds and the connection of this effect with the regulation of the antioxidant system.Item Response of Triticum aestivum seedlings of different ecological and geographical origin to heat and drought: relationship with resistance to oxidative stress and osmolyte accumulation(2023) Kolupaev, Yu. E.; Yastreb, T. O.; Ryabchun, N. I.; Kuzmyshyna, N. V.; Shkliarevskyi, M. A.; Barabolia, O. V.; Pysarenko, V. M.; Бараболя, Ольга Валеріївна; Писаренко, Віктор МикитовичThe phenomenon of plant cross-tolerance to various stressors, particularly heat and drought, has been studied in considerable detail. However, there are no data on the relationship between resistance to these stressors in Triticum aestivum cultivars of different ecological and geographical origins at the stage of etiolated seedlings. At the same time, they are used for an accelerated assessment of the heat and drought resistance of breeding samples (separately for each factor). This work compared the response of seedlings of seven winter common wheat cultivars to heat stress (4-hour heating at 45°C) and a model drought (action of 12% PEG 6000). A correlation was found between the inhibition of seedling biomass accumulation as a whole (r=0.55) and separately for shoots (r=0.66) under heat and osmotic stress. A high correlation was shown between inhibition of shoot growth and accumulation of hydrogen peroxide and lipid peroxidation (LPO) products in shoots during heating (r=0.91 and 0.76, respectively) and a much lower correlation between the values of these markers of oxidative stress and inhibition of shoot growth during drought. A significant inverse correlation was found between the accumulation of sugars in the shoots and inhibition of shoot growth under drought (r=-0.85), and moderately high under heat stress (r=-0.60). At the same time, only a positive medium correlation (r=0.49) was observed between proline content and growth inhibition under both types of stress. However, a high positive correlation was found between proline and LPO products under drought conditions (r=0.91). It is concluded that the resistance of wheat seedlings to oxidative stress is more closely related to heat tolerance than to drought tolerance. The results also indicate a significant contribution of sugars, but not proline, to the resistance of wheat seedlings to drought and heat stress.