Друковані видання. Кафедра паразитології та ветеринарно-санітарної експертизи

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https://dspace.pdau.edu.ua/handle/123456789/5089

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Перегляд Друковані видання. Кафедра паразитології та ветеринарно-санітарної експертизи за Автор "Dolhin, O. S."

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  • Ескіз
    Публікація
    Disinvasive activity of modern disinfectants against Ascaridia galli nematode eggs
    (2025) Melnychuk, V. V.; Мельничук, Віталій Васильович; Kovalenko, O.; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Zamaziy, A. A.; Замазій, Андрій Анатолійович; Dolhin, O. S.; Долгін, Олександр Сергійович
    Maintaining the well-being of poultry farms, increasing the number of birds, as well as enhancing their productivity depends on the implementation of therapeutic and preventive measures. Compliance with zoohygienic requirements and veterinary and sanitary rules for keeping chickens ensures veterinary well-being of farms, including with regard to nematodoses of the digestive tract. The aim of the research was to determine the disinvasive activity of modern disinfectants against eggs of the nematode Ascaridia galli, which parasitizes chickens. Under laboratory conditions, the destructive effect of domestically produced disinfectants was determined, including "Kristal-900" (active ingredients: glutaraldehyde, glyoxalaldehyde, benzalkonium chloride), "Creolin" (cresols, naphthalene, resin acids, pyridine bases and emulsifiers) and "Desirex Forte ParvoStop" (quaternary ammonium compounds, glutaraldehyde, non-inogenic surfactants) on a test culture of ascarid eggs isolated from the gonads of female nematodes. The conducted studies have established a high level of disinvasive effectiveness against A. galli nematode eggs of the following agents: "Krystal-900" – at 1.5 % concentration with exposure times of 1 h, 2 h and 3 h (90.3 %, 94.0 % and 100.0 % respectively); "Creolin" – at 4.0 % concentration for exposures of 2 and 3 h (92.9 and 100.0 % respectively) and at 5.0 % concentration for all exposure periods (100.0 %); "Desirex Forte ParvoStop" – at 0.5 % concentration with exposure times of 2 and 3 h (93.7 and 100.0 % respectively). Lower concentrations of the tested disinfectants, depending on the exposure duration, had either satisfactory or unsatisfactory levels of disinvasion efficiency against the eggs of the nematode A. galli. The destructive effect of disinfectants was accompanied by changes in the morphological structure of the eggs, which were characterized by the arrest of their development and loosening of the embryo; destruction and loosening of the eggshell, disintegration of both the embryo and shell, and larval death. The obtained research results allow us to recommend the disinfectants "Krystal-900", "Creolin" and "Desirex Forte ParvoStop" in specific application regimens for the effective control and prevention of ascaridiosis in chickens
  • Ескіз
    Документ
    The influence of chemicals on the development and viability of Trichuris vulpis nematode eggs isolated from infested dogs
    (2023) Євстаф’єва, Валентина Олександрівна; Долгін, Олександр Сергійович; Мельничук, Віталій Васильович; Дедухно, Алла Володимирівна; Піщаленко, Марина Анатоліївна; Красота, Олена Григорівна; Yevstafieva, V. O.; Dolhin, O. S.; Melnychuk, V. V.; Dedukhno, A. V.; Pishchalenko, M. A.; Krasota, O. G.
    Preventive measures against invasive diseases should be carried out taking into account the peculiarities of the life cycle of helminths.This is especially relevant for geohelmintiases, where the infection is mainly transmitted via environmental objects contaminated with the pathogen at the exogenous stages of the parasite’s development. It has been proven that among the causative agents of nematodoses of the gastrointestinal tract of animals, the eggs of geohelminths of the genus Trichuris are the most resistant to the action of disinfectants. The aim of the research was to establish in laboratory conditions the ovicidal effect of chemicals on the development and viability of Trichuris vulpis nematode eggs isolated from infested dogs. Two disinfectants were tested in vitro, Arquades-plus (dialkyl dimethyl ammonium chloride, dodecyl dimethyl ammonium chloride, tetrasodium salt) and Brovadez-plus (alkyl dimethyl benzyl ammonium chloride, dodecyl dimethyl ammonium chloride). Arquades-plus disinfectant showed a high level of ovicidal efficiency (94.0%) only at the maximum concentration and exposure (2.0%, 60 min), and 4.7% of nematode eggs in the test culture remained viable. Brovadez-plus disinfectant was less effective and did not provide a high level of ovicidal efficiency against Trichuris eggs in the studied concentrations and exposures. Satisfactory and unsatisfactory levels of its ovicidal effect (63.1–77.1% and 2.5–58.9%) were established when using the product in concentrations of 2.0% and 0.5–1.5%, respectively, for exposures of 10–60 min. The disinfestation activity of the preparations was characterized by metric changes in experimental test cultures compared to the control test culture. Under the influence of Arquadesplus at all concentrations, the length of the eggs was greater (by 0.4–2.0%), and the width was smaller (by 0.7–4.9%). Under the influence of Brovadez-plus in concentrations of 1.0–2.0%, the length of the eggs was greater (by 0.5–0.9%), the width was smaller (by 0.3–4.1%). The results of the conducted studies allow us to recommend the Arquades-plus disinfectant in a 2.0% concentration (exposure of 60 minutes) for effective control and prevention of infection of dogs with the causative agent of trichurosis