Друковані видання. Кафедра паразитології та ветеринарно-санітарної експертизи

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A panel of five microsatellites as an alternative to mitogenome sequencing for investigating the genetic diversity of Echinococcus granulosus sensu stricto from global to farm scale
(2026) Umhang, G.; Bastid, V.; Rinaldi, L.; Pepe, P.; Ciccone, E.; Sotiraki, S.; Ligda, P.; Oudni-M’rad, M.; M’rad, S.; Lahmar, S.; Said, Y.; Elmehatli, K.; Ahmed, H.; Kuzmina, T.; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Aghayan, S. A.; Gevorgyan, H.; Simsek, S.; Ponce-Gordo, F.; Benchikh El Fegoun, M. C.; Berbri, I. E.; Fihri, O. F.; Saarma, U.; Grenouillet, F.; Bou´, F.; Aguayo, J.
While the molecular identification of Echinococcus granulosus species is essential for surveillance, a detailed investigation of the parasite’s genetic diversity would offer a more accurate understanding of its transmission. This may be done by partial or whole mitochondrial genome sequencing, but both cost and time requirements limit routine application. Microsatellites are an alternative approach as they are a rapid, simple, highly discriminative, and low-cost tool. Our research project was designed to obtain a panel of microsatellite targets for population genetic analyses to describe the genetic diversity of E. granulosus sensu stricto (s.s.) at the global to farm scale. A panel of five informative microsatellite loci for population genetic analysis of E. granulosus s.s. was developed and validated. A total of 145 isolates from 11 countries were used to evaluate the panel at the international level. Additionally, 131 samples from sheep and cattle across six regions in southern Italy were analyzed to assess the panel’s usefulness at both the regional and farm scale. Population genetics showed high genotypic diversity. Simpson’s index gave high values (0.94–0.98) for the expected heterozygosity (0.80 to 0.87). Discriminant analysis of principal components (DAPC) revealed the structure of populations for each country, with Greece distinctly distant from Algeria, Italy, and Tunisia. This was also confirmed by FST values. DAPC further revealed a genetic structure among the farms from the Italian regional sample set, with three different clusters explained by different farming practices. This microsatellite panel can provide data relevant for E. granulosus s.s. population genetics from global to farm scale, making it a low-cost, simple alternative to full mitochondrial genome sequencing for analyzing numerous samples and obtaining a detailed overview of genetic diversity, an input that is useful for cystic echinococcosis control programs
Current state of soil microbiota in the Zaporizhzhia region of Ukraine
(2025) Melnychuk, V. V.; Мельничук, Віталій Васильович; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Bilan, M.; Zazharskyi, V.; Zazharska, N.; Davydenko, P.; Plys, V.; Shapran, I.; Yakovenko, V.; Zamaziy, A. A.; Замазій, Андрій Анатолійович
The destruction of the Kakhovka HPP led to large-scale changes in the natural ecosystems of the Zaporizhia and Kherson regions of Ukraine, including changes in the soil microbiome. The research in the territory of Zaporizhia Oblast revealed natural, intensive phenomena of soil cover purification from organic matter contamination, including feces, occurring in the drainage zone formed as a result of the destruction of the dam. Sanitary state control allows us to assess the safety of soils for the environment and determine the possible impact on humans. Thus, a local contamination was revealed. The sanitary state of the soil was characterized by the presence of sanitary indicator microorganisms (Escherichia coli, Enterococcus spp., Klebsiella spp., Enterobacter spp., Citrobacter spp., and Clostridium perfringens), which indicated fresh or old fecal contamination. The detection of thermophilic bacteria, Bacillus spp., and saprophytic fungi was correlated with the degree of organic matter transformation processes and stages of soil self-purification. In the soils in unsatisfactory sanitary condition, the coli titer the perfringens titer measured ≤ 0.9 and ≤ 0.009, respectively. Pathogenic Salmonella spp. were not detected in any of the soil samples, yet Mycobacterium spp. were isolated. In the soils of the drainage zone of most of the studied transects, the presence of E. coli and sulfite-reducing bacteria was established. The results obtained can be used for further monitoring and control of the sanitary condition of soils affected by the destruction of the Kakhovka HPP in order to prevent the spread of pathogenic microorganisms in natural ecosystems
Description of feather mite Proctophyllodes musicus(Acariformes, Proctophyllodidae) obtained from Turdus merula
(2024) Мельничук, Віталій Васильович; Melnychuk, V. V.; Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Коломак, Ігор Олегович; Kolomak, I. O.; Філоненко, Сергій Васильович; Filonenko, S. V.; Опара, Надія Миколаївна; Opara, N. M.; Muzyka, D.; Chaplygina, A.
Feather mites (Acariformes, Astigmata) are a large group of species in which mites of the genus Proctophyllodes Robin, 1868. Analgoidea are among the most common parasites of passerines. These mites are permanent obligate ectoparasites localized on the flight feathers, greater wing coverts, and on the tail. Most species of feather mites are characterized by high host specificity. The taxonomy and fauna of feather mites of the genus Proctophyllodes, and their morphological identification characters in different countries of the world are still a matter of research. The aim of our work was to describe the morphological features and metric parameters of feather mites of the species P. musicus Vitzthum, 1922, isolated from the common blackbird Turdus merula Linnaeus, 1758 in Ukraine. 32 parameters are determined in males and females of P.musicus mites, which characterize both their general structure of the body and sexual dimorphism. The structure and sizes of the body, gnathosoma, idiosoma, prodorsal and hysteronotal shields, epimerites I, setae cp and c3, and the distance between setae of the scapula se–se and si–si are described. In males, the sizes of adanal sucker discs, epigastric shields, genital arch, setae ps2, h3, h2, the distance between setae 4a–4a, g–g, ps3–ps3, ps1–ps1 are additionally described and determined. Lamellae in males are wide, tongueshaped, do not overlap. The aedeagus is short, slightly extending beyond the posterior margin of the genital arch. In female mites, the dimensions of the lobar shield, terminal appendages, and terminal recess are additionally described and determined, taking into account the location of setae ps1, h2, the dimensions of setae cp, c3, h3, h2, the distance between setae h1–h1, the distance between hysteronotal and lobar shields. The mite eggs were measured in the body of the female and on the feathers of the birds. The scientific data obtained in this study expand the already existing data on morphological and metric characters of feather mites of the species P. musicus(Proctophyllodidae) isolated from the common blackbird
DESCRIPTION OF THE NEMATODE DIROFILARIA REPENS (NEMATODA, ONCHOCERCIDAE) PARASITIC IN DOGS IN UKRAINE
(2023) Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Кравченко, Сергій Олександрович; Kravchenko, S. O.; Киричко, Олена Борисівна; Kyrychko, O. B.; Дмитренко, Надія Іванівна; Dmytrenko, N. I.; Сизоненко, Наталія Миколаївна; Syzonenko, N. M.; Опара, Надія Миколаївна; Opara, N. M.
Dirofi lariasis is a zoonotic disease of domestic and wild carnivorous animals and humans. Subcutaneous migration of the parasite Dirofi laria repens Railliet & Henry, 1911 can lead to the local swellings with changeable localization. In humans, this parasite can cause the damage of lungs, male genitalia, and eyes. Th e range of D. repens is constantly spreading to new areas, and, considering the zoonotic potential of this parasite, it is important to widen the existing data concerning the morphology of D. repens. Present study provides the morphometric description of nematodes D. repens, obtained from subcutaneous tissue of dogs in Ukraine. Th e aim of this study was to make an accent on the key features for performing the identifi cation of parasites of this species. For males, 27 morphometric traits were identifi ed, characterising the total size of the body and the esophagus, the thickness of the cuticle in the region of the mouth opening, the localisation of the nerve ring and the cloaca, the morphological structure and metric parameters of the spicules, and the length of the tail. For females, 18 morphometric traits were identifi ed that, in addition to the common parameters, characterise cuticle thickness in the region of the vulva, localisation of the vulva and anus, body width in the regions of the anus and vulva, and tail length. Th is report widens the existing data on the morphological and metric characteristics of nematodes D. repens
Differential characters of fleas of the genus Ctenocephalides (Siphonaptera, Pulicidae) obtained from dogs
(2021-05) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Горб, К.О.; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Горб, Олег Олександрович
Differential species characters of Baruscapillaria anserisand B. obsignatanematodes obtained from the domestic goose
(Regulatory Mechanism in Biosystems, 2018) Yevstafieva, V. O. ; Євстаф’єва, Валентина Олександрівна; Yeresko, V.I.; Pishchalenko, M. A.; Nagorna, L. V.; Піщаленко, Марина Анатоліївна
The study presents species composition and abundance of nematodes of the family Capillariidae (Nematoda, Trichocephalida), parasitizing the domestic goose (Anser anser dom. Linnaeus, 1758) in Poltava region, Ukraine. Morphological specifics of adult specimens of collected helminths are examined and new data is obtained for species identification of Capillariidae considering their metric parameters. The pathogens of capillariasis in geese are represented by two species, Baruscapillaria anseris (Madsen, 1945) Moravec, 1982 and B. obsignata(Madsen, 1945) Moravec, 1982 with the former predominant. Prevalence of infection by B. anserisin domestic fowl was up to 46.8%, the abundance index reached 17.9 specimens.
Disinvasive activity of modern disinfectants against Ascaridia galli nematode eggs
(2025) Melnychuk, V. V.; Мельничук, Віталій Васильович; Kovalenko, O.; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Zamaziy, A. A.; Замазій, Андрій Анатолійович; Dolhin, O. S.; Долгін, Олександр Сергійович
Maintaining the well-being of poultry farms, increasing the number of birds, as well as enhancing their productivity depends on the implementation of therapeutic and preventive measures. Compliance with zoohygienic requirements and veterinary and sanitary rules for keeping chickens ensures veterinary well-being of farms, including with regard to nematodoses of the digestive tract. The aim of the research was to determine the disinvasive activity of modern disinfectants against eggs of the nematode Ascaridia galli, which parasitizes chickens. Under laboratory conditions, the destructive effect of domestically produced disinfectants was determined, including "Kristal-900" (active ingredients: glutaraldehyde, glyoxalaldehyde, benzalkonium chloride), "Creolin" (cresols, naphthalene, resin acids, pyridine bases and emulsifiers) and "Desirex Forte ParvoStop" (quaternary ammonium compounds, glutaraldehyde, non-inogenic surfactants) on a test culture of ascarid eggs isolated from the gonads of female nematodes. The conducted studies have established a high level of disinvasive effectiveness against A. galli nematode eggs of the following agents: "Krystal-900" – at 1.5 % concentration with exposure times of 1 h, 2 h and 3 h (90.3 %, 94.0 % and 100.0 % respectively); "Creolin" – at 4.0 % concentration for exposures of 2 and 3 h (92.9 and 100.0 % respectively) and at 5.0 % concentration for all exposure periods (100.0 %); "Desirex Forte ParvoStop" – at 0.5 % concentration with exposure times of 2 and 3 h (93.7 and 100.0 % respectively). Lower concentrations of the tested disinfectants, depending on the exposure duration, had either satisfactory or unsatisfactory levels of disinvasion efficiency against the eggs of the nematode A. galli. The destructive effect of disinfectants was accompanied by changes in the morphological structure of the eggs, which were characterized by the arrest of their development and loosening of the embryo; destruction and loosening of the eggshell, disintegration of both the embryo and shell, and larval death. The obtained research results allow us to recommend the disinfectants "Krystal-900", "Creolin" and "Desirex Forte ParvoStop" in specific application regimens for the effective control and prevention of ascaridiosis in chickens
Ectoparasites Ctenocephalides (Siphonaptera, Pulicidae) in the composition of mixed infestations in domestic dogs from Poltava, Ukraine
(2020-07) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Горб, К.; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Бахур, Т. І.; Фещенко, Д.
Effect of Dermanyssus gallinae on zootechnical and productive indicators of farming chicken.
(2024) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Петруненко, А. П.
Results of the study of the impact of ticks on the productive indicators of industrial poultry farming in Ukraine are presented in the article. The spread of parasites that attack poultry leads to significant losses in the poultry industry. Modern technologies of poultry farming create favourable conditions for the rapid spread of dermanisiosis in chickens. Dermanyssus gallinae ticks are among the most dangerous blood-sucking ectoparasites, in addition, they are carriers of dangerous bird infections pathogens. The goal of the work was to establish the effect of D. gallinae parasitism on zootechnical indicators of chickens. The research was carried out on the premicies of the "Poultry Farms of Poltava region" farm in the Poltava district of the Poltava region with the support of the laboratory of parasitology of the Poltava State Agrarian University (Poltava). The results of present study established detrimental impact of D. gallinae parasitism on zootechnical indicators of chickens (total number of birds, survival rate, live weight, livestock losses) during the poultry farming. In particular, under the conditions of contamination of the poultry house, the birds were dying before the age of 59 weeks, and the live weight of chickens with D. gallinae ectoparasites was 32.1% lower during this period compared to the clinically healthy birds. In clinically healthy chickens, live weight gradually increased from 1.4 to 2.2 kg from 15 weeks to 34 weeks. While in birds contaminated with ectoparasites, the weight increased from 1.3 to 1.9 kg during the same period, but gradually decreased from the 34th week to 1.4 kg at the 58th week. The survival rate of tick-contaminated chickens during the experiment decreased to 24.9% (over a period of 58 weeks) and was 73.9% lower than that of clinically healthy chickens aged 58 weeks. It was established that the death rate of chickens under the conditions of D. gallinae tick parasitism was 75.0%, which is 7.2 times higher than that of clinically healthy chickens. The data we obtained prove the relevance of research and development of means of solving the problem of chicken dermanisiosis in Ukraine, given the significant negative impact of ectoparasites D. gallinae on the live weight of chickens, the period of their breeding, and the indicators of their survival rates
Effect of temperature on the survival rates of the embryonic states of development of Trichuris skrjabini nematodes parasitizing sheep
(2023-06) Yevstafieva, V. O.; Petrenko, M. O.; Melnychuk, V. V.; Vakulenko, Y. V.; Titarenko, O. V.; Shaferivskyi, B. S.; Pishchalenko, M. A.; Filonenko, S. V.; Sheiko, S. V.; Євстаф’єва, Валентина Олександрівна ; Петренко, Максим Олександрович ; Мельничук, Віталій Васильович ; Вакуленко, Юлія Валентинівна; Бахур-Каваляускене, Т. І.; Тітаренко, Олена Вікторівна ; Шаферівський, Богдан Сергійович ; Піщаленко, Марина Анатоліївна ; Філоненко, Сергій Васильович ; Шейко, Сергій Володимирович
The prevention of the trichurosis of sheep requires understanding the terms of development of pathogens and their survival in the environment under the influence of abiotic factors. This study was conducted to determine the tolerance to a temperature factor of Trichuris skrjabini nematode eggs isolated from sheep. Trichuris eggs were obtained from the gonads of female nematodes in laboratory conditions and cultivated in a thermostat until the appearance of motile larvae in the eggs at the temperatures of 20°C, 25°C, and 30°C. It was determined that the time of formation of infectious eggs decreases with increasing temperature. The temperature of 25°С was found to be the most favorable for the development of T. skrjabini eggs in laboratory conditions, and 80.3% of eggs with motile larvae formed on the 54th day. Temperatures of 20°С and 30°С were less advantageous to the process of embryogenesis. The motile larvae develop in eggs in 63 days at 20°С and in 45 days at 30°С, and their respective survival rates are 77.0% and 75.3%. The obtained data increase the effectiveness of planning measures to prevent the trichurosis of sheep, taking into account the period of development of the pathogen depending on the temperature of the environment
Effectiveness of the application of an improved method of laboratory coproscopic diagnostics of toxocarosis in dogs
(2025) Melnychuk, V. V.; Yevstafieva, V. O.; Kitichenko, A.; Євстаф’єва, Валентина Олександрівна; Мельничук, Віталій Васильович; Кітіченко, А. С.
Toxocara canis is a nematode that causes toxocarosis in dogs, which has zoonotic potential. T. canis larvae are transmitted transplacentally from females to newborn puppies and, after a complex hepatopulmonary migration, develop into sexually mature helminths in the small intestine. In young and adult dogs, after alimentary ingestion of parasite eggs, larvae hatch, migrate to skeletal muscles, kidneys, liver and heart and persist for years as somatic larvae. Somatic larvae in the tissues of females are activated during pregnancy and are a reservoir of invasion
Efficacy of treatment measures for rabbit passalurosis
(2023) Хорольський, А. А.; Євстаф’єва, Валентина Олександрівна; Мельничук, Віталій Васильович; Yevstafieva, V. O.; Melnychuk, V. V.
Helminthes of Passalurus ambiguus species are one of the most common parasites of the intestinal tract of domestic rabbits both on industrial rabbit farms and private farms. The most effective way to treat and prevent passalurosis in animals at present is the use of anti-helminthic preparations, where determining the degree of their anti-parasitic effect is a topical direction of research. This will make it possible to raise the effectiveness of maintaining well-being on rabbit farms and the animals’ timely recovery from this infestation. The purpose of the study was to establish the effectiveness of treatment measures for rabbit passalurosis. The research was carried out on a private farm that was unfavorable as to passalurosis and on the basis of the Laboratory of Parasitology of Poltava State Agrarian University. The therapeutic efficacy of Brovermectin 1 % solution (the active substance is ivermectin), Brovalzen powder (the active substance is albendazole), Albendazole 7.5 % suspension (the active substance is albendazole) was tested in case of rabbit spontaneous passalurosis. It was established that on the 7th day of treatment, the indicators of extense-effectiveness and intense-effectiveness were, respectively: of Brovermectin 1 % – 50.0 and 64.31 %, Brovalzen powder – 70.0 and 82.63 %, Albendazole 7.5 % suspension – 60.0 and 60.9 %. On the 14th day of treatment, Brovalzen powder and Albendazole 7.5 % suspension turned out the most effective preparations for rabbit passalurosis, where the indicators of their extense-effectiveness and intenseeffectiveness reached 100.0 %. A lower therapeutic effectiveness was registered when using Brovermectin 1 % solution, which was injected to diseased animals. Its extense-effectiveness and intense-effectiveness made 60.0 and 80.9 %, respectively. Moreover, the indicators of the intensity of passalurous invasion on the 7th and 14th day of treatment were at the level of 4.20±1.27 and 2.50±0.75 eggs, and the indicators of the prevalence of passalurous infection were 50.0 and 40.0 %, respectively. The obtained results of experimental studies allow recommend antihelminthic oral preparations of domestic production Brovalzen powder and Albendazole 7.5 % suspension for effective treatment and prevention of rabbit passalurosis
Epizootological monitoring studies of chicken heterakosis spreading on the territory of Poltava region
(2023) Євстаф’єва, Валентина Олександрівна; Омельченко, Ольга Вікторівна; Yevstafieva, V. O.; Omelchenko O. V.
Poultry breeding is one of the most promising branches of agriculture. Serious losses to this industry are caused by invasion diseases, including chicken heterakosis, which is widespread throughout the world. Heterakises cause pathological changes in the caecum, where various forms of typhlitises and enterohepatitises develop, and at high prevalence of infection, especially in young animals, lead to their death. The causative agent of the disease, Heterakis gallinarum nematode, is a parasitic organism environmentally adapted to the climatic conditions of many geographical zones, which contributes to its wide spreading. The purpose of the research was to investigate chicken heterakosis spreading based on the results of analyzing the reporting documentation of the State Food and Consumer Service in Poltava region during 2018–2023. The conducted monitoring studies have established that in the conditions of the investigated region, the share of heterakosis among contagious poultry diseases makes 7.62 %. Infective poultry diseases account for 8.57 %. At the same time, the share of invasion diseases among poultry virulent pathology reaches 83.81 %. The share of heterakosis among poultry invasion diseases was at the level of 8.33 %, where the share of protozoases was the largest (67.71 %). Nematodoses accounted for a smaller share (18.75 %). Cestodiases were most rarely detected in poultry (5.21 %). Among the protozoan, nematodous and cestodous poultry diseases on the territory of Poltava region, eimeriosis made the largest percentage (67.71 %). Among nematodoses, the share of ascariasis was 16.67 %, capillariasis – 2.08 %. Among cestodoses, drepanidoteniosis was detected, the share of which made 5.21 %. Analyzing the indicators of the prevalence of heterakosis infection found on the territory of poultry farms in Poltava region, it was found that chickens’ infestation rate with heterakosis pathogen ranged from 0.06 to 0.19 % with an average value of 0.1 %. The highest indicators of the prevalence of heterakosis infection were detected in 2019–0.19 %. During 2020–2022, the infestation rates of poultry with heterakises gradually decreased from 0.12 to 0.06 %. The results of monitoring studies point to the relevance of further investigating the epizootological peculiarities of chicken heterakosis on the territory of certain regions of Ukraine
Evaluation of effectiveness of disinfectants against exogenous stages of development of Nematodirus spathiger nematodes
(2025) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Пономаренко, В. М.; Ponomarenko, V. M.; Melnychuk, V. V.; Мельничук, Віталій Васильович; Suprunenko, K. V.; Супруненко, Костянтин Володимирович; Karysheva, L. P.; Каришева, Людмила Павлівна; Nikiforova, O.; Нікіфорова, О. В.; Krykunova, V. E.; Крикунова, Валентина Юхимівна; Mezhenska, N. A.; Меженська, Н. А.
One of the most common nematode diseases of ruminants is gastrointestinal strongyliasis. The pathogen’s life cycle partly occurs in the external environment. Its propagative stages are eggs secreted by infested animals, and invasive larvae, which persist in the environment for a long time and form biological pollution. Therefore, an integral part of the effective control and prevention of gastrointestinal strongyliasis of ruminants is the implementation of measures aimed at destroying pathogens in the environment to curb their potential distribution. The aim of the research was to investigate the disinvasive effectiveness of chemical preparations against eggs and invasive L3 larvae of the nematode Nematodirus spathiger, isolated from cattle, in laboratory conditions. It is established that the products "Hermecid-VS" (contains didecyldimethylammonium chloride, glutaraldehyde, benzalkonium chloride) and "Virosan" (contains benzalkonium chloride and glutaraldehyde) have ovicidal and larvicidal properties against exogenous stages of development of N. spathiger. At the same time, "Hermecid-VS" showed higher disinvasion activity compared to "Virosan". The ovicidal activity of disinfectants was higher than the larvicidal one. High levels of ovicidal efficacy were established when "Hermecid-VS" was used : 90.9% at a 0.1% concentration (exposure 60 min), and 100.0% at 0.25% and 0.5% concentrations (10–60 min). The larvicidal efficiency of "Hermecid-VS" was 90.7% at 0.25% concentration (60 min), and 95.3–100.0% at 0.5% concentration (10–60 min). The preparation "Virosan" showed a high level of ovicidal efficacy when used at a concentration of 0.25% (30–60 min) – 92.6–100.0%, at a concentration of 0.5% (10–60 min) – 100.0%, and a high level of larvicidal efficacy at a concentration of 0.5% (60 min) – 97.0%. The detrimental effect of disinfectants was characterized by morphological changes in the eggs and L3 larvae of N. spathiger, as well as in the metric parameters of N. spathiger eggs during their development. The obtained research results allow us to recommend the disinfectants "Hermecid-VS" and "Virosan" with the mentioned usage protocols to increase the effectiveness of prevention and treatment of N. spathiger infection in cattle
Features of the clinical course of canine cystoisosporosis
(2025) Melnychuk, V. V.; Мельничук, Віталій Васильович; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Suvorov, R.; Суворов, Р.
The conducted studies have established the features of the clinical course of cystoisosporosis in dogs with an invasion intensity of up to 500 oocysts/g, taking into account the severity of symptoms. A total of 13 clinical symptoms were identified, which have a slightly pronounced manifestation, and 6 - are expressed to a significant extent. The most frequent symptoms with a slight intensity of invasion are thirst (up to 30.77 %), decreased appetite and dullness of the coat (up to 23.08 %), abdominal wall tenderness (up to 20.51 %). Diarrhea was detected in 10.26 % of dogs
Forticept® innovative products’ effectiveness for complex udder hygiene
(2020-12) Шевченко, А. Н.; Фещенко, Д. В.; Бахур, Т. І.; Згозинська, О. А.; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Сашук, Р. Н.; Стравський, Є. С.; Антіпов, А. А.; Melnychuk, V. V.; Мельничук, Віталій Васильович
Impact of military actions on the epizootic situation with the spread of rabies in animals in Kherson Oblast
(2024) Мельничук, Віталій Васильович; Melnychuk, V. V.; Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Слинько, Віктор Григорович; Slynko, V. H.
Rabies is a zoonotic disease caused by a neurotropic RNA virus of the Lyssavirus genus, recorded in various species of wild and domestic animals in many countries of the world, including Ukraine, where this disease has been continuosly diagnosed in animals and sporadically in people. Therefore, there is a need to study the epizootic specifics of rabies in different species of animals as potential sources of threat to people in individual administrative-territorial units of Ukraine. This becomes especially relevant against the background of martial law and the catastrophic flooding which took place in Kherson Oblast. Determining the peculiarities and manifestations of epizootic process of rabies in animals was conducted in the conditions of de-occupied territories of Kherson Oblast in 2023. During that year, 27 studies of samples of pathological/biological materials from animals suspected of rabies were conducted. Those included 24 samples from domestic animals – cattle (Bos taurus), dogs (Canis lupus familiaris), cats (Felis silvestris catus), and 3 from wild animals – fox (Vulpes vulpes) and jackals (Canis aureus). According to the results, rabies was confirmed in 88.9% of the total number of analyzed samples. Most often, rabies was diagnosed in domestic animals, particularly, dogs and cats –45.8% and 29.2%, respectively. In the representatives of wild fauna, rabies was detected in 12.5% of the cases, in particular, 8.3% in foxes and 4.2% in jackals. The analysis of the seasonality of rabies morbidity in animals found no pattern in the dynamics. However, according to species, the peak of rabies in foxes took place in autumn, particularly, September and October. An important fact is that the bulk of rabies cases, acccounting for 91.7%, occurred in the period after the Kahovka Dam had been blown up by the Russian Federation. Territorially, during the surveillance period, rabies in animals was confirmed in 23 settlements of Kherson Oblast: 22 cases within the Oblast’s districts and one case in the city of Kherson proper
In vitro tests of effect of disinfectants on the viability of Heterakis gallinarum nematode eggs during embryogenesis
(2024) Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Омельченко, Ольга Вікторівна; Omelchenko O. V.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Дмитренко, Надія Іванівна; Dmytrenko, N. I.; Крикунова, Валентина Юхимівна; Krykunova, V. E.; Передера, Олена Олександрівна; Peredera, O. O.; Тагільцева, Яніна Михайлівна; Tahiltseva, Y. М.
Affecting the transmission of an infection, objects of the external environment become contaminated with pathogens as a result of their massive release by the definitive host during exogenous stages of parasites’ development, which is an important chain of their epizootic process. Therefore, disinfestation plays an important role in the complex of measures for the prevention and control of poultry helminthiases, including Heterakis gallinarum infection of chickens. The purpose of the research was to investigate in laboratory conditions the ovicidal efficiency of modern disinfectants in relation to the viability of developing eggs of H. gallinarum isolated from infested chickens. "Hermecid-VS" (didecyldimethylammonium chloride, glutaraldehyde, benzalkonium chloride) and "Arquadez-plus" (dimethyldialkylammonium chloride, didecyldimethylammonium chloride, tetrasodium salt) disinfectants were tested. These preparations had a high level of ovicidal efficiency against the eggs of Heterakis, "Hermecid-VS" in 0.25% and 0.5% concentration for exposures of 10–60min (93.5–100.0%), "Arquadez-plus" in 1.0% concentration for exposure of 60 min (93.1%), and at 1.5% (96.8–100.0%) and 2.0% (100.0%) concentrations regardless of exposure. The ovicidal effect of disinfectants was characterized by morphological changes in the nematode eggs when using "Hermecid-VS" – accumulation of air bubbles under the shell, thinning and deformation of the shell, death and gradual resorption of the embryo, loosened egg shell and terminated embryonic development, deformation and thinning of egg the shell, and death of the embryo at the stage of formation of the larva; under the effect of "Arquadez-plus", a stop in development at the stage of cleaving blastomeres, destruction of the shell, death and evacuation of the embryo from the egg, shrinkage and gradual defragmentation of the embryo, death of the larva and its defragmentation. Under the action of disinfectants, the metric parameters of Heterakis eggs also changed. Under the influence of "Hermecid-VS" at all concentrations, the length of the eggs and the thickness of the shell were larger (by 2.9–5.4% and 26.7–46.7%), and their width was smaller (by 5.5–6.9%). With the effect of "Arquadez-plus", the length of the eggs was greater (by 0.4–2.0%), and the width was smaller (by 0.7–4.9%) only when using 0.1–2.0% concentrations of the agent. Heterakis egg shell thickness increased under the influence of "Arquadez-plus" in concentrations of 0.25–2.0% (by 33.3–40.0%). The conducted studies allow us to recommend "Hermecid-VS" and "Arquadez-plus" disinfectants in specified concentrations and modes of their use for maintaining epizootic well-being in the complex of measures against Heterakisinfection of chickesn
Influence of Ctenocephalides felis on biochemical indicators of blood serum of infested cats
(2025) Гаврик, Б. А.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Havryk, B. А.
Fleas represent one of the most important groups of blood-sucking parasitic insects. An important aspect of the medical and veterinary importance of fleas, which has not lost its relevance even today, is their role in maintaining natural foci of zooanthroponotic and zoonotic infections. With constant flea attacks on an animal, clinical signs develop, characterized by itching, anxiety, and dermatopathologies, which are due to certain parasite-host relationships, which are based on the interaction of the pathogenic effect of the parasite and the host's defense mechanism. The aim of the research was to determine changes in the biochemical parameters of blood serum of infested cats at different levels of ctenocephalic invasion intensity. The conducted studies have established that flea parasitism on the body of cats led to a negative impact of ectoparasites on the biochemical parameters of the blood serum of infested cats. With increasing intensity of flea infestation of cats, the severity of changes in the biochemical parameters of their blood serum increased. Thus, at low levels of ctenocephalic invasion intensity (up to 19 specimens/head), a decrease in albumin content by 3.99 % (p<0.05) was detected in the blood serum of infected cats compared to the levels in clinically healthy cats. All other indicators were within physiological fluctuations. At higher rates of intensity of ctenocephalic invasion (from 20 to 42 specimens/head) in the blood serum of infected cats, a decrease in albumin content by 15.39 % (p<0.01), glucose by 35.99 % (p<0.05), an increase in total bilirubin content by 33.54 % (p<0.05), urea by 36.24 % (p<0.05), creatinine by 16.18 % (p<0.05), as well as an increase in the activity of enzymes alanine aminotransferase by 22.42 % (p<0.05), aspartate aminotransferase by 38.56 % (p<0.05), alkaline phosphatase by 2.4 times (p<0.05), α-Amylase by 51.44 % (p<0.05), gamma-glutamyl transpeptidase by 31.22 % (p<0.05) compared to the indicators in clinically healthy cats. The results obtained expand existing data on the impact of fleas on the body of infested cats, and also allow us to take into account indicators of infestation intensity in increasing the effectiveness of treatment measures
Influence of Tempetature on Viability and Deveplopment of Heterakis gallinarum (Nematoda, Heterakidae) Eggs. Zoodiversity
(2024) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Омельченко, Ольга Вікторівна; Omelchenko O. V.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Нагорна, Людмила Володимирівна; Nagorna, L. V.; Петренко, Максим Олександрович; Petrenko, M. O.; Шаферівський, Богдан Сергійович; Shaferivskyi, B. S.; Кравченко, Сергій Олександрович; Kravchenko, S. O.; Супруненко, Костянтин Володимирович; Suprunenko, K. V.; Шейко, Сергій Володимирович; Sheiko, S. V.; Каришева, Людмила Павлівна; Karysheva, L. P.; Бурда, Тетяна Леонідівна;; Burda, T. L.; Сизоненко, Наталія Миколаївна; Syzonenko, N. M.; Савенкова, Олена Олександрівна; Savenkova, O. O.; Люлька, Вікторія Миколаївна; Liulka, V. M.
Pinworms of the genus Heterakis Dujardin, 1845 parasitize a wide range of hosts, including a number of species of domestic and wild birds. Th ese nematodes are ecologically adapted to the climatic conditions of many geographical zones, which contributes to their widespread distribution. Th e aim of the work was to investigate the infl uence of diff erent temperature regimes at laboratory conditions on the growth, development and viability of eggs isolated from the gonads of female nematodes Heterakis gallinarum Schrank, 1788. Th e conducted studies established that, depending on the cultivation temperature, nematode eggs developed from 6 to 56 days, and their survival rate was from 67.7 to 83.7 %. Th e temperature of 25 °С was the most favourable for the development of H. gallinarum eggs, the development lasted 12 days and the highest number of eggs with motile infective larvae was formed (83.7 %). It was found that the period of egg development in cultures gradually decreased with increasing temperature: it was 56 days at 15 °C, 18 days at 20 °C, 12 days at 25 °C and 6 days at 30 °C. Th e survival rate of H. gallinarum eggs gradually increased with increasing temperature in regimes of 15 °С, 20 °С and 25 °С to 67.7 %, 80.7 % and 83.7 %, respectively. At a temperature of 30 °C, egg survival decreased slightly to 78.7 %. It was found that the development of H. gallinarum eggs from the zygote stage to the formation of motile infective larvae, depending on the temperature regime of cultivation, is accompanied by a decrease in egg length by 4.9–5.4 %, an increase in egg width by 4.1–7.6 % and a thinning of the shell by 9.5–28.6 %. Under diff erent temperature regimes, the morphometric parameters of eggs during their development diff er in terms of their length, width and shell thickness

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