Друковані видання. Кафедра паразитології та ветеринарно-санітарної експертизи

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    Evaluation of effectiveness of disinfectants against exogenous stages of development of Nematodirus spathiger nematodes
    (2025) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Пономаренко, В. М.; Ponomarenko, V. M.; Melnychuk, V. V.; Мельничук, Віталій Васильович; Suprunenko, K. V.; Супруненко, Костянтин Володимирович; Karysheva, L. P.; Каришева, Людмила Павлівна; Nikiforova, O.; Нікіфорова, О. В.; Krykunova, V. E.; Крикунова, Валентина Юхимівна; Mezhenska, N. A.; Меженська, Н. А.
    One of the most common nematode diseases of ruminants is gastrointestinal strongyliasis. The pathogen’s life cycle partly occurs in the external environment. Its propagative stages are eggs secreted by infested animals, and invasive larvae, which persist in the environment for a long time and form biological pollution. Therefore, an integral part of the effective control and prevention of gastrointestinal strongyliasis of ruminants is the implementation of measures aimed at destroying pathogens in the environment to curb their potential distribution. The aim of the research was to investigate the disinvasive effectiveness of chemical preparations against eggs and invasive L3 larvae of the nematode Nematodirus spathiger, isolated from cattle, in laboratory conditions. It is established that the products "Hermecid-VS" (contains didecyldimethylammonium chloride, glutaraldehyde, benzalkonium chloride) and "Virosan" (contains benzalkonium chloride and glutaraldehyde) have ovicidal and larvicidal properties against exogenous stages of development of N. spathiger. At the same time, "Hermecid-VS" showed higher disinvasion activity compared to "Virosan". The ovicidal activity of disinfectants was higher than the larvicidal one. High levels of ovicidal efficacy were established when "Hermecid-VS" was used : 90.9% at a 0.1% concentration (exposure 60 min), and 100.0% at 0.25% and 0.5% concentrations (10–60 min). The larvicidal efficiency of "Hermecid-VS" was 90.7% at 0.25% concentration (60 min), and 95.3–100.0% at 0.5% concentration (10–60 min). The preparation "Virosan" showed a high level of ovicidal efficacy when used at a concentration of 0.25% (30–60 min) – 92.6–100.0%, at a concentration of 0.5% (10–60 min) – 100.0%, and a high level of larvicidal efficacy at a concentration of 0.5% (60 min) – 97.0%. The detrimental effect of disinfectants was characterized by morphological changes in the eggs and L3 larvae of N. spathiger, as well as in the metric parameters of N. spathiger eggs during their development. The obtained research results allow us to recommend the disinfectants "Hermecid-VS" and "Virosan" with the mentioned usage protocols to increase the effectiveness of prevention and treatment of N. spathiger infection in cattle
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    Influence of Tempetature on Viability and Deveplopment of Heterakis gallinarum (Nematoda, Heterakidae) Eggs. Zoodiversity
    (2024) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Омельченко, Ольга Вікторівна; Omelchenko O. V.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Нагорна, Людмила Володимирівна; Nagorna, L. V.; Петренко, Максим Олександрович; Petrenko, M. O.; Шаферівський, Богдан Сергійович; Shaferivskyi, B. S.; Кравченко, Сергій Олександрович; Kravchenko, S. O.; Супруненко, Костянтин Володимирович; Suprunenko, K. V.; Шейко, Сергій Володимирович; Sheiko, S. V.; Каришева, Людмила Павлівна; Karysheva, L. P.; Бурда, Тетяна Леонідівна;; Burda, T. L.; Сизоненко, Наталія Миколаївна; Syzonenko, N. M.; Савенкова, Олена Олександрівна; Savenkova, O. O.; Люлька, Вікторія Миколаївна; Liulka, V. M.
    Pinworms of the genus Heterakis Dujardin, 1845 parasitize a wide range of hosts, including a number of species of domestic and wild birds. Th ese nematodes are ecologically adapted to the climatic conditions of many geographical zones, which contributes to their widespread distribution. Th e aim of the work was to investigate the infl uence of diff erent temperature regimes at laboratory conditions on the growth, development and viability of eggs isolated from the gonads of female nematodes Heterakis gallinarum Schrank, 1788. Th e conducted studies established that, depending on the cultivation temperature, nematode eggs developed from 6 to 56 days, and their survival rate was from 67.7 to 83.7 %. Th e temperature of 25 °С was the most favourable for the development of H. gallinarum eggs, the development lasted 12 days and the highest number of eggs with motile infective larvae was formed (83.7 %). It was found that the period of egg development in cultures gradually decreased with increasing temperature: it was 56 days at 15 °C, 18 days at 20 °C, 12 days at 25 °C and 6 days at 30 °C. Th e survival rate of H. gallinarum eggs gradually increased with increasing temperature in regimes of 15 °С, 20 °С and 25 °С to 67.7 %, 80.7 % and 83.7 %, respectively. At a temperature of 30 °C, egg survival decreased slightly to 78.7 %. It was found that the development of H. gallinarum eggs from the zygote stage to the formation of motile infective larvae, depending on the temperature regime of cultivation, is accompanied by a decrease in egg length by 4.9–5.4 %, an increase in egg width by 4.1–7.6 % and a thinning of the shell by 9.5–28.6 %. Under diff erent temperature regimes, the morphometric parameters of eggs during their development diff er in terms of their length, width and shell thickness