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Involvement of Nitric Oxide in γ-Aminobutyric Acid-Induced Cellular Mechanisms of Wheat Seedling Adaptation to Water Deficit

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dc.contributor.authorShakhov, I. V.
dc.contributor.authorYastreb, T. O.
dc.contributor.authorSakhno, T. V.
dc.contributor.authorСахно, Тамара Вікторівна
dc.contributor.authorKolupaev, Y. E.
dc.date.issued2025
dc.descriptionShakhov I. V., Yastreb T. O., Sakhno T. V., Kolupaev Y. E. Involvement of Nitric Oxide in γ-Aminobutyric Acid-Induced Cellular Mechanisms of Wheat Seedling Adaptation to Water Deficit. Cytol. Genet. 2025. Vol. 59. P. 580–595. DOI: https://doi.org/10.3103/S0095452725060106
dc.description.abstractγ-Aminobutyric acid (GABA) is one of the key stress metabolites involved in the functioning of the plant cell signaling network. However, its functional relationships with main signaling molecules, particularly nitric oxide (NO), are not well understood. This study aimed to determine NO’s involvement in GABA’s stress-protective effect on wheat seedlings (Triticum aestivum L., Etana cultivar) under model drought con ditions (13% PEG 6000). Priming grains with a 0.5 mM GABA solution or a 0.1 mM solution of the NO donor sodium nitroprusside (SNP) increased germination energy, seed germination, and water content in seedlings under stressful conditions. Additionally, GABA and SNP treatment mitigated the inhibitory effect of drought on the accumulation of root and shoot biomass. Osmotic stress increased NO content in shoots, and priming with GABA and SNP enhanced this effect. Treatment with the nitric oxide scavenger methylene blue (MB, 0.1 mM) eliminated the increase in NO content caused by stress or GABA action. MB also negated the positive effects of GABA on growth processes. Under the action of GABA and SNP, total amylase activity in grains and soluble carbohydrate content in shoots increased under stressful conditions; MB eliminated GABA’s effect on these parameters. Under the model drought, the content of oxidative stress markers (super oxide anion radical, hydrogen peroxide, and malondialdehyde) increased in shoots, but pretreatment of grains with GABA or SNP significantly reduced these effects. Osmotic stress caused an increase in activity of catalase and guaiacol peroxidase in shoots. Preliminary priming with GABA, SNP, and MB did not affect the nature of the changes in activity of these antioxidant enzymes caused by model drought. Meanwhile, super oxide dismutase activity remained unchanged following exposure to the model drought; however, pretreat ment seeds with GABA, SNP, and MB increased the enzyme activity. Priming seeds with GABA and SNP increased the total content of phenolic compounds in shoots and preserved the anthocyanin pool under stressful conditions, while MB treatment eliminated these effects. It was concluded that the enhancement of grain germination and seedling growth induced by GABA priming under model drought conditions is largely due to NO-mediated modulation of carbohydrate and secondary compound metabolism.
dc.identifier.urihttps://dspace.pdau.edu.ua/handle/123456789/22134
dc.language.isoen
dc.subjectγ-aminobutyric acid
dc.subjectnitric oxide
dc.subjectoxidative stress
dc.subjectantioxidant system
dc.subjectosmolytes
dc.subjectamylase
dc.subjectdrought resistance
dc.subjectTriticum aestivum
dc.titleInvolvement of Nitric Oxide in γ-Aminobutyric Acid-Induced Cellular Mechanisms of Wheat Seedling Adaptation to Water Deficit
dc.typeArticle
dspace.entity.typePublication
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Міжнародні видання. Навчально-науковий інститут агротехнологій, селекції та екології
Друковані видання. Кафедра землеробства і агрохімії ім. В.І.Сазанова