(Acta fytotechn zootechn, 2025) Ilchenko, M. O.; Ільченко, Марія Олександрівна
Using the polymerase chain reaction method in real-time, we investigated the polymorphism of the RYR1 and LEP genes in sows of the sizeable white breed of different origins. Following the purpose of the work, we conducted a study of the genetic structure of the above-mentioned genes in animals of French and Belgian breeds and their crossbreeds. No RYR1 gene polymorphism was detected. All experimental animals had the homozygous NN genotype. According to the genetic structure of the LEP gene, a significant difference has been established between animals of different origins. Animals of French breeding had a high frequency of the TT genotype, while in animals of other origins, this genotype did not occur at all. Accordingly, French breeding animals are characterized by a high frequency of the T allele and Belgian and crossbred animals are characterized by the A allele. In French breeding animals, there is a violation of the distribution of genotypes, according to Hardy-Weinberg. Obtaining animals of the desired genotypes among the experimental herd is possible due to the use of breeders with the appropriate genotypes for the studied genes.