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Публікація Abscisic acid and biopreparations as markers of organic soybean adaptation to hydrothermal stress(2025) Chaika, T.; Korotkova, I. V.; Liashenko, V. V.; Pospielova, G. D.; Onipko, V. V.; Чайка, Т.; Короткова, Ірина Валентинівна; Ляшенко, Віктор Васильович; Поспєлова, Ганна Дмитрівна; Оніпко, Валентина ВолодимирівнаGiven the growing demand for organic soybeans and the need to mitigate the adverse effects of climate change, the study of biopreparations of various origins and their influence on plant adaptive mechanisms under stress conditions has become increasingly relevant. This study aimed to assess changes in abscisic acid (ABA) content under the influence of biopreparations in a multi-year field experiment and to analyse the relationship between ABA and the biochemical, physiological, and agronomic indicators of soybean adaptation to hydrothermal stress under organic farming conditions. The study involved the use of biological preparations based on mycorrhizal fungi, rhizosphere and nitrogen-fixing bacteria, as well as phytohormones. A range of field, laboratory (physiological and biochemical), and statistical methods was employed. The findings revealed the integration of molecular, biochemical, physiological, and agronomic stress resistance markers as a result of the enhanced adaptive potential of soybean following the application of biopreparations. Their effectiveness was evident through a comprehensive influence on key adaptive mechanisms, including: a 9.2%-36.3% reduction in ABA concentration, indicating alleviation of stress pressure; a 10.0%-27.6% increase in relative water content, contributing to the maintenance of optimal cellular water balance; a 19.2%-65.4% improvement in stomatal conductance, attributed to the optimisation of stomatal function; a 15.6%-41.8% reduction in lipid peroxidation levels (malondialdehyde), indicating effective membrane protection against oxidative stress; a 9.1%-46.6% increase in proline content, suggesting the activation of natural defence mechanisms and the maintenance of osmotic balance. Correlation analysis confirmed that the biopreparations facilitated a complex modulation of the plant stress response, optimising the balance between defence reactions and productivity, which led to a 12.3%-45.5% increase in yield even under adverse weather conditions. It was established that under water stress, the biological preparations modulated ABA biosynthesis and its regulatory role, contributing to coordinated changes in osmoprotective mechanisms, antioxidant defence, and the plant water regime. The most effective approach proved to be the triple combination of biopreparations, which ensured an optimal balance between phytohormonal regulation, physiological processes, and soybean productivity under hydrothermal stress. The results obtained may be recommended for the cultivation of organic soybeans to improve yield under climate-related challenges.Документ Agro-ecological territories zoning for the cultivation of organic agricultural products.(2017) Ласло, Оксана Олександрівна; Писаренко, Павло ВікторовичДокумент Agrobiological features and use of cornflower (Centaurae cyanus L.)(2022-05-20) Запорожець, Владислав Костянтинович; Поспєлов, Сергій ВікторовичДокумент Agrobiological features of St. John’s wort (Hypericum perforatum L.)(2022-05-20) Семенко, Максим Васильович; Поспєлов, Сергій ВікторовичПублікація Agroclimatic Conditions as the Main Factor in Realizing the Productive Potential of Sunflower(2026-06-08) Laslo, O. O.; Ласло, Оксана ОлександрівнаThe influence of agro climatic indicators on the formation of oilseed productivity, in particular sunflower, is a determining factor in the stability of agricultural production. In conditions of climate change, the rational selection of hybrids that are able to maximize their genetic potential in specific soil and climatic conditions becomes particularly relevantПублікація Application of quantum-chemical methods to estimate the carcinogenic properties of benzopyrene metabolites(2024) Хахель, Олег Альбінович; Khakhel, O. A.; Ромашко, Таміла Петрівна; Romashko, T. P.Benzo[a]pyrene is a common environmental pollutant and a powerful carcinogen that can accumulate in various organs and tissues, causing DNA damage through the adducts’ formation, which contributes to the development of neoplasms of various localization. This compound carcinogenic activity is well studied and proven to be related to the nature and electronic configuration of biologically activated metabolites. Since not all metabolites of benzo[a]pyrene show carcinogenic effects, in this study an attempt to theoretically investigate their spectral properties and to use the obtained data in establishing a correlation with the observed carcinogenic effect was made. In order to study the spectroscopic properties of benzo[a]pyrene metabolites which, depending on the transformation pathways, can form products with different carcinogenic activity, the energies of the lower electronically excited states of metabolite molecules were calculated using the semi-empirical PM3 method (Hyper Chem 7.5). To determine the features of the electron density distribution during the molecules’ excitation, we used the values of the occupancy of the highest occupied molecular orbital and the lowest unoccupied molecular orbital, which participate in one-electron transitions. The calculation results of the position of the benzo[a]pyrene absorption spectra satisfactorily correlate with the experimental data presented in the literature, which is a confirmation of the obtained data reliability regarding the spectral characteristics of its metabolites. The performed calculations made it possible to determine the position of the long-wavelength absorption bands of some metabolites, for which experimental data have not been currently determined. It has been established that the long wave bands in the absorption spectra of carcinogenic metabolites of benzo[a]pyrene in the number: BP → BP 7,8-epoxide → BP 7,8-dihydrodiol → BP 7,8-dihydrodiol-9,10-epoxide are equally intense and sequentially shifted to the short-wavelength region relative to the benzo[a]pyrene absorption spectra.Публікація Assessing the efficiency of bacterial and phytohormonal soybean (Glycine max L.) seed treatment under organic farming technology(2025-12-26) Короткова, Ірина Валентинівна; Korotkova, I. V.; Chaika, T.; Shevnikov, M.; Liashenko, V.; Gorbenko, O.; Rybalchenko, A.Soybean (Glycine max L.) is one of the most sought-after legumes on the global market due to its high demand in the feed, food, and industrial sectors. Climate change necessitates the implementation of technological innovations to enhance plant resistance to adverse growing conditions. The aim of this study was to investigate the effect of pre-sowing seed treatment of soybean with the bacterial preparation Profix, the phytohormone based preparation Violar, as well as their combined application through seed inoculation and crop spraying during the bud formation–bloom phase, on the development and productivity of soybeans under an organic farming. To examine the dynamics of leaf surface area, photosynthetic pigments, malondialdehyde and proline levels, and their relationship with yield, the ANOVA-Tukey test and principal component analysis were employed. The field experimental results showed that pre-sowing seeds treatment with the Profix inoculant, the phytohormonal preparation Violar, and their combined application contributes to an increase in leaf surface area, an enhancement in the content of photosynthetic pigments, a reduction in malondialdehyde levels, and an increase in proline content in soybean plant. These physiological improvements ultimately led to average soybean yield increases of 12.3%, 19.6%, and 29.2%, respectively, despite adverse weather conditions 2024. These results demonstrate the effectiveness of integrating bacterial and phytohormonal seed treatments as a sustainable and innovative approach to enhancing soybean productivity under organic farming conditions.Публікація Assessment of landfills and their impact on the soil: a local study in Ukraine(2024) Писаренко, Павло Вікторович; Pysarenko, P. V.; Samojlik, M.; Писаренко, Віктор Микитович; Pysarenko, V. M.; Mostoviak, I.; Тараненко, Анна Олексіївна; Taranenko, A. O.; Тараненко, Сергій Володимирович; Taranenko, S. V.; Диченко, Оксана Юріївна; Dychenko, O. Yu.; Lastovka, V.; Husinsky, D.Landfills are widely utilised for waste disposal due to their economic advantages and ease of implementation compared to alternative methods. However, landfills exert significant environmental and health impacts on adjacent agricultural land. Accumulation of heavy metals in soil is a risk to ecological and food safety. Methodological approaches to assess and mitigate the impact of landfills on agricultural land are essential for ensuring sustainable land use practices and safeguarding human health. In this study, landfills were assessed at the local level, and the hazard level was classified according to it. A set of priority measures for restoring technogenic disturbed areas and minimising their impact on agricultural land was determined. The need to select a set of innovative, ecologically oriented methods for remediation of landfills, depending on the type and degree of soil contamination, was identified in context of ensuring environmental and food security.Публікація Assessment of the soil microbial community under energy crops (Panicum virgatum L. and Miscanthus giganteus): a case study from Ukraine(2025) Тараненко, Анна Олексіївна; Taranenko, A. O.; Тараненко, Сергій Володимирович; Taranenko, S. V.; Кулик, Максим Іванович; Kulyk, M. I.; Rytchenko, A.; Teteriuk, R.Bioenergy crops are a promising alternative for energy production. They can be grown on inaccessible, degraded, marginal land that is not economically viable for traditional agriculture. At the same time, a broader evaluation of the environmental and ecological impact of energy crop cultivation on land is needed. There are still knowledge gaps regarding the mechanisms underlying soil carbon accumulation, especially concerning the involvement of the soil microbiome in facilitating these processes during energy crop cultivation. The present study determined the main ecotrophic and taxonomic groups of soil microbial communities and the direction of soil microbiological processes under Panicum virgatum L. and Miscanthus giganteus growth. Research results showed an initial impact of energy crop growth on soil microbial communities in the soil-climatic conditions of Ukrainian forest-steppe zones. Soils under energy crops are characterized by the highest abundances of the investigated ecological and trophic microbial groups (by 1.2-3 times) compared to control (soil under grassland). The coefficients of microbiological processes determine the high potential for increasing soil fertility under energy crop cultivation. Statistical analysis of the results confirmed a medium and strong correlation between soil microbiological parameters and soil organic carbon content during energy crop growth. Therefore, energy crops can act as ecosystem engineers, improving soil biological and chemical properties and supporting soil ecosystem sustainability.Публікація Assessment of the soil microbial community under energy crops (Panicum Virgatum L. and Miscanthus x Giganteus): a case study in Ukraine(2025) Тараненко, Анна Олексіївна; Taranenko, A. O.; Тараненко, Сергій Володимирович; Taranenko, S. V.; Кулик, Максим Іванович; Kulyk, M. I.; Rytchenko, A.; Teteriuk, R.Bioenergy crops are a promising alternative for energy production. They can be grown on inac cessible, degraded, marginal land that is not economically viable for traditional agriculture. At the same time, a broader evaluation of the environmental and ecological impact of energy crop cultiva tion on land is needed. There are still knowledge gaps regarding the mechanisms underlying soil carbon accumulation, especially concerning the involvement of the soil microbiome in facilitating these processes during energy crop cultivation. The present study determined the main ecotrophic and taxonomic groups of soil microbial communities and the direction of soil microbiological proc esses under Panicum virgatum L. and Miscanthus giganteus growth. Research results showed an initial impact of energy crop growth on soil microbial communities in the soil-climatic conditions of Ukrainian forest-steppe zones. Soils under energy crops are characterized by the highest abundanc es of the investigated ecological and trophic microbial groups (by 1.2–3 times) compared to control (soil under grassland). The coeffi cients of microbiological processes determine the high potential for increasing soil fertility under energy crop cultivation. Statistical analysis of the results con f i rmed a medium and strong correlation between soil microbiological parameters and soil organic carbon content during energy crop growth. Therefore, energy crops can act as ecosystem engineers, improving soil biological and chemical properties and supporting soil ecosystem sustainability.Публікація Biological mechanisms of invasiveness of Ambrosia artemisiifolia L. and innovative control approaches(2025-12-03) Оніпко, Валентина Володимирівна; Onipko, V. V.; Поспєлов, Сергій Вікторович; Pospelov, S. V.; Поспєлова, Ганна Дмитрівна; Pospielova, G. D.; Pospelova, H; Коваленко, Нінель Павлівна; Kovalenko, N. P.The purpose of the review was to summarize current scientific approaches to studying the biological mechanisms of invasiveness of Ambrosia artemisiifolia L., to identify ecological and agroeconomic risks associated with its spread, and to analyze innovative strategies for controlling this dangerous weed. Ambrosia artemisiifolia L. is one of the most aggressive invasive species of the Asteraceae family, posing a significant threat to agroecosystems, natural biodiversity, and human health. Its spread under conditions of trade globalization and climate change has reached a large scale, emphasizing the need for interdisciplinary research on the bioecological characteristics of this species and the development of comprehensive control strategies. The high invasive potential of A. artemisiifolia is determined by a complex of adaptive traits: rapid initial growth, tolerance to various soil types and climatic conditions, a powerful root system, cross-pollination, and pronounced seed heteromorphism. These features ensure the formation of persistent soil “seed banks” that support long-term population recovery even after mechanical or agronomic interventions. In addition, the considerable genetic variability of the species promotes local adaptation to changing climatic and anthropogenic factors, complicating control efforts across regions. It was emphasized that the expansion of ragweed in Europe and Asia leads to reduced crop yields, displacement of native species, and ecosystem degradation. Losses in the agricultural sector are associated with both direct decreases in the productivity of maize, sunflower, soybean, and other crops, and with increasing costs of herbicide application. However, chemical control is complicated by the development of resistance to major groups of herbicides. This underlines the relevance of integrated weed management approaches, that combine agronomic practices (crop rotation, optimization of sowing density, cultivation of competitive crops), biological control (entomophages, phytopathogens, microbial preparations), chemical methods, and environmentally safe technologies. A promising direction is the use of modern geographic information systems, remote sensing methods, and mathematical modeling to monitor the spread of A. artemisiifolia and predict its invasion dynamics under climate change. Such an approach makes it possible to identify priority control areas and effectively allocate resources. Further research should focus on improving adaptive integrated management systems for ragweed populations, assessing the long-term consequences of its invasion for biodiversity and human health, and developing ecologically oriented models of sustainable land use.Публікація Comparative characteristics of morphometric indicators of quarantine species of the genus Cuscuta (Cuscuta L.) growing in Ukraine(2025-12-08) Коваленко, Нінель Павлівна; Kovalenko, N. P.; Поспєлова, Ганна Дмитрівна; Pospielova, G. D.; Самородов, Віктор Миколайович; Samorodov, V. M.; Поспєлов, Сергій Вікторович; Pospelov, S. V.; Pospielov, S.; Оніпко, Валентина Володимирівна; Onipko, V. V.A review is devoted to the comparative characteristics of the morphometric and biological features of doddlers in Ukraine based on a scientific source base. The systematic position of the common quarantine parasitic plants of the genus Cuscuta L. (dodder), commonly found in Ukraine, is presented. A morphometric analysis of 15 species is provided, highlighting differences in stem diameter, length, and color, features of the flower structure, types of inflorescences, and fruit shape. The literature data indicate a high degree of similarity among Cuscuta species prevalent in our country, which significantly complicates their identification in natural environments and agrocenoses, and is insufficient for clear and, more importantly, unmistakable identification by professionals in applied branches of the agricultural industry. Knowledge of the biological characteristics of Cuscuta species is important for understanding their spread and high naturalization potential. Currently, this information is quite fragmented and insufficiently studied. Information is presented regarding the most studied species, namely: C. campestris Yunck., C. trifolii Bab., C. europaea L., C. epilinum Weihe, C. Lemanniana Bge., C. lupuliformis Krock. and C. epithymym L. An important factor in the formation of the infectious background in agrocenoses and natural environments is the seed viability period. It has been established that the longest period of preservation of its viability is observed in C. trifolii Bab. (10-12 years); up to 6 years in C. campestris Yunck, C. europaea L., and C. Lemanniana Bge. The shortest viability period is up to one year (in the soil) in C. epilinum Weihe. However, there are reports about the possibility of preserving seed viability for up to 25–30 years under conditions unfavorable for its germination. In our opinion, this is due to such a phenomenon as "hardseededness". The formation of the seed bank in the soil is also influenced by the fertility of the species. Among the Cuscuta species growing in Ukraine, this indicator has been established only for seven species: C. campestris Yunck., C. trifolii Bab., C. europaea L., C epilinum Weihe, C. Lemanniana Bge., C. lupuliformis Krock., and C. epithymym L. The largest number of seeds per plant is produced by C. Lemanniana Bge. (up to 100.000) and C. campestris Yunck. (up to 114.000). The period of emergence of seedlings has been found to be March-May. It depends on the soil temperature and the depth of seed placement. It has been determined that at a soil temperature of 16–25 ⁰С, mass seed germination occurs in May-June. The flowering period is extended and lasts from June to August.Публікація Correction of sowing quality of medicinal plants seeds with echinaceia extracts(2024) Pospielov, S. V.; Mishchenko, O. V.; Onipko, V. V.; Pospielova, G. D.; Поспєлов, Сергій Вікторович; Міщенко, Олег Вікторович; Оніпко, Валентина Володимирівна; Поспєлова, Ганна ДмитрівнаThe paper presents experimental data on the effect of echinacea extracts on the sowing qualities of medicinal herbs. The biological activity of extracts obtained from the areal part (EAP) and rhizomes with roots (ERS) of pale echinacea was investigated. The object of study was the sowing quality of the seeds of such medicinal plants as blue cornflower (Centaurea cyanus L.), purple echinacea (Echinacea purpurea (L. Moench.), pale echinacea (Echinacea pallida (Nutt.) Nutt.), St. John's wort (Hypericum perforatum L.), forest mallow (Malva sylvestris L.), which are of industrial importance for medicinal plants. The seeds were soaked in 0.01%-0.0001% solutions of extracts, under the control they were treated with water, after that they were germinated in Petri dishes by generally accepted methods according to the standards. The best results were obtained in mallow seed extracts. The solution of EAP at a concentration of 0.001% increased the energy of germination by 19.2% compared to the control, and when using ERS in all concentrations, a positive effect was obtained (by 18.2 %-23.6% to the control). Germination of variants treated with EAP solutions increased by 1.12-1.21 times, and ERS – by 1.09-1.21 times. Treatment of Echinacea purple seeds with EAP solutions was not effective, but rhizome extract treatment with roots had a positive effect on both germination energy and germination resulting 12.0%-17.3% and 8.3%-9.5% compared to the control, respectively. Similar regularities were observed in variants with the treatment of Echinacea seeds with extracts in different concentrations. The action of EAP solutions did not statistically exceed the control, but when using ERS, the results reliably exceeded the control by 10.8%-13.8% (germination energy) and by 11.0%- 17.8% (germination). Treatment of St. John's wort and cornflower seeds with extracts did not lead to changes in seed quality indicators. A general trend of greater activity of extracts in concentrations of 0.01%-0.001% was observed.Документ Dependence of growth processes of Echinacea purpurea (L.) Moench. of the first year of vegetation on agrometeorological factors(2022-05-20) Поспєлов, Сергій Вікторович; Поспєлова, Ганна Дмитрівна; Поспєлов, Ілля СергійовичДокумент Dynamics of soil organic matter in Panicum virgatum sole crops and intercrops(2021) Taranenko, A. O. ; Kulyk, M. I. ; Galytska, M. A. ; Taranenko, S. V.; Rozhko, I. I. ; Тараненко, Анна Олексіївна; Кулик, Максим Іванович; Галицька, Марина Анатоліївна; Тараненко, Сергій Володимирович; Рожко, Ілона ІванівнаSoil organic matter (SOM) is one of the primary indicators of soil quality, which plays an important role in enhancing a range of soil physical, chemical and biological functions in the agricultural ecosystem. Switchgrass cultivation for bioenergy has the potential to improve and stabilize soil properties on marginal land over the years. The research was aimed to evaluate the dynamics of SOM in different types of switchgrass cropping systems and the influence of SOM on switchgrass productivity. The experiment was performed on a marginal land in the central part of the forest-steppe zone of Ukraine. The switchgrass (Panicum virgatum L.) cultivar ‘Cave-in-Rock’ was used. The experiment included two factors. Factor A – type of cropping: 1) sole crops of switchgrass (Sw) – control; 2) strip intercropping of switchgrass and lupine (Sw + strip); 3) mixed intercropping of switchgrass and lupine (Sw + mix). Factor B – years of switchgrass cultivation (2010–2016). It was demonstrated that perennial switchgrass cultivation has the potential to increase SOM content. SOM content in Sw + strip crops increased by 0.12%, in Sw + mix – by 0.09% and in Sw – by 0.07%. The highest switchgrass productivity was formed in Sw + strip (from 1.11 to 1.53 kg m-2) and in Sw + mix (from 1.12 to 1.45 kg m-2) crops, the lowest – in Sw (from 0.85 to 1.34 kg m-2) crop. The maximum switchgrass productivity was observed for 5–7 years of cultivation. The highest height of plants and number of shoots of switchgrass were in Sw + strip crops, and significantly less in the other treatments. The results of the experiment showed a great increase of switchgrass productivity and SOM content in switchgrass crops and confirmed that switchgrass can be a sustainable energy crop.Документ Effect of grain meteorological factors and time of spring vegetation recovery on the development and yield of winter grain crops in Ukraine.(2014) Калініченко, Антоніна Володимирівна; Гангур, Володимир Васильович; Маренич, Микола Миколайович; Kalinichenko, A. V.; Hanhur, V. V.; Marenych, M. M.Публікація Effect of probiotic treatment on the microbiological activity of Ukrainian typical black soil(2024) Писаренко, Павло Вікторович; Pysarenko, P. V.; Samoilik, M.; Тараненко, Анна Олексіївна; Taranenko, A. O.; Тараненко, Сергій Володимирович; Taranenko, S. V.; Bybyk, Y.The search for novel substances that promote the establishment of a microbial community and facilitate optimal humification processes while increasing soil organic matter content offers an opportunity for land restoration. The objective of study was to determine the abundance of ecological and trophic groups of soil microorganisms and the intensity of microbiological processes when subjected to probiotic treatment at various concentrations and doses in typical black soil. The eco-trophic groups of soil microorganisms were identified by inoculating dilutions of soil suspensions onto selective nutrient media. The direction and intensity of soil microbiological processes were assessed using the mineralization-immobilization, oligotrophy, and pedotrophy indexes. The results demonstrate that probiotics has a positive impact on the microbiological activity of the soil, leading to an increase in the number of ecological and trophic groups of soil microorganisms during both spring and autumn seasons. Notably, a significant effect on the soil microorganism conditions was observed after 30 days of probiotic treatment. Probiotics exhibit a favorable influence on microbiological processes within the soil, fostering conducive conditions for the development of soil microorganisms and the formation of humus. The most effective concentration of probiotics for promoting the functionality of microbial communities in black soils is determined to be 10%, accompanied by a dose of 100 l ha-1. Consequently, the application of probiotic treatment at a concentration of 10% and a dose of 100 l/ha-1 holds the potential to enhance the biological state of the soil, restore soil microbial diversity, and serve as an environmentally safe fertilizer.Публікація Effect of the water origin on the biological properties of sage (Salvia officinalis L.) aqueous extracts(2025) Короткова, Ірина Валентинівна; Korotkova, I. V.; Ромашко, Таміла Петрівна; Romashko, T. P.; Хахель, Олег Альбінович; Khakhel, O. A.; Zvenihorodska, T.; Япринець, Тетяна Сергіївна; Yaprynets, T. S.; Ляшенко, Віктор Васильович; Liashenko, V. V.Plant extracts, due to the high content of various biologically active compounds, have found their wide application in the foods, pharmaceutical industries, and agricultures, for the production of valuable and useful bioproducts. The aqueous plant extracts are most often used. The effect of the water origin used in their preparation on the extract’s composition has not been studied enough, which limits their use. The study was aimed to compare the biological effect of sage (Salvia officinalis L.) extracts prepared using water of different origins (distilled, tap, spring, bottled). The preparation of aqueous extracts was carried out in two ways, differing Keywords in the extraction temperature. In the first method, boiling water was used to prepare the extract; in the second, cooled boiled water was used. The sage leaves mass for extract preparation was 1.65 and 3.30 g. The biological activity of the extracts was assessed by testing them for radish germination. It was shown the sage leaves extracts prepared using various waters origin can differ by one and half times in their allelopathic effect on radish seeds. It was proved the qualitative composition and mass of the substance extracted from the plant depend on the water origin used for extracts preparation. Thus, using the same amount of plant material to prepare extracts, you can increase their biological activity by fifty percent with the right choice of water.Публікація Efficiency of probiotic application for the remediation of contaminated soils in agrocenoses(2023) Писаренко, Павло Вікторович; Pysarenko, P. V.; Samoilik, M.; Тараненко, Анна Олексіївна; Taranenko, A. O.; Mostoviak, I.; Лавріненко, Інна Вікторівна; Lavrinenko, I.; Shpyrna, V.The problem of soil contamination is relevant today. Soil contamination is generally associated with intensive industrial activities, inadequate waste management, mining, military activities, or accidents. Pollutants (e.g. heavy metals) are accumulating in soil and have major indirect impacts on biodiversity, quality of groundwater resources, and food safety. Soil contamination of agricultural land is a particular threat. Due to the military action and other conditions, the numbers of sites where potentially polluting activities have taken place are increasing in Ukraine. The cultivation of agricultural crops on soils with a medium or high level of toxicity is only possible after the re mediation of this area. The use of biological methods to intensify soil remediation processes, thereby reducing the additional burden on the environment, is becoming more widespread. The application of probiotics is an innova tive and ecological method for the restoration of contaminated soils. This will enable to improve the conceptual approaches to the implementation of an ecologically safe model for the restoration of contaminated land under the conditions of military action in Ukraine. The aim of the research was to assess the phytotoxic effect of soil con taminated with heavy metals and petroleum products before and after probiotic application. The seedling method was used to determine the remediation potential of contaminated soils. An express test of Triticum aestivum was used to determine soil phytotoxicity. The research results show the negative impact of soil contamination with heavy metals and petroleum products on the biometric indices of Triticum aestivum plants and positive dynamics of biometric indices of Triticum aestivum in variants after probiotic treatment (86–92% compared to the control). The obtained data show that a significant phytotoxic effect is observed by all biometric indices of Triticum aesti vum in all studied variants. The highest phytotoxic effect (33.56–42.70%) was observed in variants with combined contamination (PP+Zn+Pb). The results of probiotic application show a phytotoxic effect of less than 20% by all biometric indices of Triticum aestivum for all studied variants. Therefore, the results of the research can be used to develop recommendations for the remediation of land contaminated by military actions in Ukraine and the creation of sustainable agroecosystems.