Browsing by Author "Melnychuk, V. V."
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Item An Investigation of Embryo and Eggshell Development in Trichuris suis (Nematoda, Trichuridae) under Laboratory Conditions(2016-06-15) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Юськів, І. Д.; Melnychuk, V. V.; Мельничук, Віталій ВасильовичItem Comparative embryonic development of nematodes of the genus Тrichuris (Nematoda, Trichuridae) obtained from sheep (Ovis aries)(2018-11-14) Melnychuk, V. V.; Мельничук, Віталій Васильович ; Березовський, А. В.Item Comparative morphology and biology of nematodes of genus Heterakis (Nematoda, Heterakidae), parasites of the domestic goose (Anser anser) in Ukraine(2018-04-28) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Нікіфорова, О.В.; Супруненко, Костянтин Володимирович; Корчан, Леонід Миколайович; Локес-Крпука, Т.П.; Негребецький, Ігор Станіславович; Корчан, Микола Іванович; Suprunenko, K. V.Publication Description of feather mite Proctophyllodes musicus(Acariformes, Proctophyllodidae) obtained from Turdus merula(2024) Мельничук, Віталій Васильович; Melnychuk, V. V.; Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Коломак, Ігор Олегович; Kolomak, I. O.; Філоненко, Сергій Васильович; Filonenko, S. V.; Опара, Надія Миколаївна; Opara, N. M.; Muzyka, D.; Chaplygina, A.Feather mites (Acariformes, Astigmata) are a large group of species in which mites of the genus Proctophyllodes Robin, 1868. Analgoidea are among the most common parasites of passerines. These mites are permanent obligate ectoparasites localized on the flight feathers, greater wing coverts, and on the tail. Most species of feather mites are characterized by high host specificity. The taxonomy and fauna of feather mites of the genus Proctophyllodes, and their morphological identification characters in different countries of the world are still a matter of research. The aim of our work was to describe the morphological features and metric parameters of feather mites of the species P. musicus Vitzthum, 1922, isolated from the common blackbird Turdus merula Linnaeus, 1758 in Ukraine. 32 parameters are determined in males and females of P.musicus mites, which characterize both their general structure of the body and sexual dimorphism. The structure and sizes of the body, gnathosoma, idiosoma, prodorsal and hysteronotal shields, epimerites I, setae cp and c3, and the distance between setae of the scapula se–se and si–si are described. In males, the sizes of adanal sucker discs, epigastric shields, genital arch, setae ps2, h3, h2, the distance between setae 4a–4a, g–g, ps3–ps3, ps1–ps1 are additionally described and determined. Lamellae in males are wide, tongueshaped, do not overlap. The aedeagus is short, slightly extending beyond the posterior margin of the genital arch. In female mites, the dimensions of the lobar shield, terminal appendages, and terminal recess are additionally described and determined, taking into account the location of setae ps1, h2, the dimensions of setae cp, c3, h3, h2, the distance between setae h1–h1, the distance between hysteronotal and lobar shields. The mite eggs were measured in the body of the female and on the feathers of the birds. The scientific data obtained in this study expand the already existing data on morphological and metric characters of feather mites of the species P. musicus(Proctophyllodidae) isolated from the common blackbirdItem DESCRIPTION OF THE NEMATODE DIROFILARIA REPENS (NEMATODA, ONCHOCERCIDAE) PARASITIC IN DOGS IN UKRAINE(2023) Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Кравченко, Сергій Олександрович; Kravchenko, S. O.; Киричко, Олена Борисівна; Kyrychko, O. B.; Дмитренко, Надія Іванівна; Dmytrenko, N. I.; Сизоненко, Наталія Миколаївна; Syzonenko, N. M.; Опара, Надія Миколаївна; Opara, N. M.Dirofi lariasis is a zoonotic disease of domestic and wild carnivorous animals and humans. Subcutaneous migration of the parasite Dirofi laria repens Railliet & Henry, 1911 can lead to the local swellings with changeable localization. In humans, this parasite can cause the damage of lungs, male genitalia, and eyes. Th e range of D. repens is constantly spreading to new areas, and, considering the zoonotic potential of this parasite, it is important to widen the existing data concerning the morphology of D. repens. Present study provides the morphometric description of nematodes D. repens, obtained from subcutaneous tissue of dogs in Ukraine. Th e aim of this study was to make an accent on the key features for performing the identifi cation of parasites of this species. For males, 27 morphometric traits were identifi ed, characterising the total size of the body and the esophagus, the thickness of the cuticle in the region of the mouth opening, the localisation of the nerve ring and the cloaca, the morphological structure and metric parameters of the spicules, and the length of the tail. For females, 18 morphometric traits were identifi ed that, in addition to the common parameters, characterise cuticle thickness in the region of the vulva, localisation of the vulva and anus, body width in the regions of the anus and vulva, and tail length. Th is report widens the existing data on the morphological and metric characteristics of nematodes D. repensItem Differential characters of fleas of the genus Ctenocephalides (Siphonaptera, Pulicidae) obtained from dogs(2021-05) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Горб, К.О.; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Горб, Олег ОлександровичItem Ectoparasites Ctenocephalides (Siphonaptera, Pulicidae) in the composition of mixed infestations in domestic dogs from Poltava, Ukraine(2020-07) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Горб, К.; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Бахур, Т.; Фещенко, Д.Item Effect of temperature on the survival rates of the embryonic states of development of Trichuris skrjabini nematodes parasitizing sheep(2023-06) Yevstafieva, V. O.; Petrenko, M. O.; Melnychuk, V. V.; Vakulenko, Y. V.; Titarenko, O. V.; Shaferivskyi, B. S.; Pishchalenko, M. A.; Filonenko, S. V.; Sheiko, S. V.; Євстаф’єва, Валентина Олександрівна ; Петренко, Максим Олександрович ; Мельничук, Віталій Васильович ; Вакуленко, Юлія Валентинівна; Бахур-Каваляускене Т.І.; Тітаренко, Олена Вікторівна ; Шаферівський, Богдан Сергійович ; Піщаленко, Марина Анатоліївна ; Філоненко, Сергій Васильович ; Шейко, Сергій ВолодимировичThe prevention of the trichurosis of sheep requires understanding the terms of development of pathogens and their survival in the environment under the influence of abiotic factors. This study was conducted to determine the tolerance to a temperature factor of Trichuris skrjabini nematode eggs isolated from sheep. Trichuris eggs were obtained from the gonads of female nematodes in laboratory conditions and cultivated in a thermostat until the appearance of motile larvae in the eggs at the temperatures of 20°C, 25°C, and 30°C. It was determined that the time of formation of infectious eggs decreases with increasing temperature. The temperature of 25°С was found to be the most favorable for the development of T. skrjabini eggs in laboratory conditions, and 80.3% of eggs with motile larvae formed on the 54th day. Temperatures of 20°С and 30°С were less advantageous to the process of embryogenesis. The motile larvae develop in eggs in 63 days at 20°С and in 45 days at 30°С, and their respective survival rates are 77.0% and 75.3%. The obtained data increase the effectiveness of planning measures to prevent the trichurosis of sheep, taking into account the period of development of the pathogen depending on the temperature of the environmentItem Efficacy of treatment measures for rabbit passalurosis(2023) Хорольський, А. А.; Євстаф’єва, Валентина Олександрівна; Мельничук, Віталій Васильович; Yevstafieva, V. O.; Melnychuk, V. V.Helminthes of Passalurus ambiguus species are one of the most common parasites of the intestinal tract of domestic rabbits both on industrial rabbit farms and private farms. The most effective way to treat and prevent passalurosis in animals at present is the use of anti-helminthic preparations, where determining the degree of their anti-parasitic effect is a topical direction of research. This will make it possible to raise the effectiveness of maintaining well-being on rabbit farms and the animals’ timely recovery from this infestation. The purpose of the study was to establish the effectiveness of treatment measures for rabbit passalurosis. The research was carried out on a private farm that was unfavorable as to passalurosis and on the basis of the Laboratory of Parasitology of Poltava State Agrarian University. The therapeutic efficacy of Brovermectin 1 % solution (the active substance is ivermectin), Brovalzen powder (the active substance is albendazole), Albendazole 7.5 % suspension (the active substance is albendazole) was tested in case of rabbit spontaneous passalurosis. It was established that on the 7th day of treatment, the indicators of extense-effectiveness and intense-effectiveness were, respectively: of Brovermectin 1 % – 50.0 and 64.31 %, Brovalzen powder – 70.0 and 82.63 %, Albendazole 7.5 % suspension – 60.0 and 60.9 %. On the 14th day of treatment, Brovalzen powder and Albendazole 7.5 % suspension turned out the most effective preparations for rabbit passalurosis, where the indicators of their extense-effectiveness and intenseeffectiveness reached 100.0 %. A lower therapeutic effectiveness was registered when using Brovermectin 1 % solution, which was injected to diseased animals. Its extense-effectiveness and intense-effectiveness made 60.0 and 80.9 %, respectively. Moreover, the indicators of the intensity of passalurous invasion on the 7th and 14th day of treatment were at the level of 4.20±1.27 and 2.50±0.75 eggs, and the indicators of the prevalence of passalurous infection were 50.0 and 40.0 %, respectively. The obtained results of experimental studies allow recommend antihelminthic oral preparations of domestic production Brovalzen powder and Albendazole 7.5 % suspension for effective treatment and prevention of rabbit passalurosisItem Efficacy of ultrasound in diagnosis of dirofilariasis in dogs caused by Dirofilaria immitis(2022-11) Yevstafieva, V. O. ; Євстаф’єва, Валентина Олександрівна; Kryvoruchenko, D.; Melnychuk, V. V. ; Мельничук, Віталій Васильович; Nikiforova, O.; Kone, M. S. ; Коне, Мохамед Сумана; Barabolia, O. V.; Бараболя, Ольга ВалеріївнаItem Features of embryonic development of parasitic nematodes of the species Trichuris sylvilagi (Nematoda, Trichuridae)(2021-04) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна; Melnychuk, V. V.; Мельничук, Віталій Васильович; Нагорна, Людмила Володимирівна; Стибель, Володимир Володимирович; Гутий, Богдан Володимирович; Яценко, Іван Володимирович; Petrenko, M. O.; Петренко, Максим Олександрович; Нікіфорова, Ольга Василівна; Filonenko, S. V. ; Філоненко, Сергій Васильович; Savenkova, O. O.; Савенкова, Олена Олександрівна; Tahiltseva, Y. М.; Тагільцева, Яніна МихайлівнаItem Features of exogenous development of Trichuris globulosa (Nematoda, Trichuridae)(2020-10) Yevstafieva, V. O.; Melnychuk, V. V.; Kanivets, N. S.; Dmytrenko, N. I.; Karysheva, L. P.; Filonenko, S. V.; Філоненко, Сергій Васильович; Євстаф’єва, Валентина Олександрівна; Мельничук, Віталій Васильович; Каришева, Людмила ПавлівнаItem Features of the exogenic development of Passalurus ambiguus (Nematoda, Oxyuroidae) at different temperature regimes(2022-03) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Хорольський, А.; Kravchenko, S. O.; Кравченко, Сергій Олександрович ; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Нікіфорова, О.; Решетило, О.Item Forticept® innovative products’ effectiveness for complex udder hygiene(2020-12) Шевченко, А.Н.; Фещенко, Д.В.; Бахур, Т.І.; Згозинська, О.А.; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Сашук, Р.Н.; Стравський, Є.С.; Антіпов, А.А.; Melnychuk, V. V.; Мельничук, Віталій ВасильовичPublication Impact of military actions on the epizootic situation with the spread of rabies in animals in Kherson Oblast(2024) Мельничук, Віталій Васильович; Melnychuk, V. V.; Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Слинько, Віктор Григорович; Slynko, V. H.Rabies is a zoonotic disease caused by a neurotropic RNA virus of the Lyssavirus genus, recorded in various species of wild and domestic animals in many countries of the world, including Ukraine, where this disease has been continuosly diagnosed in animals and sporadically in people. Therefore, there is a need to study the epizootic specifics of rabies in different species of animals as potential sources of threat to people in individual administrative-territorial units of Ukraine. This becomes especially relevant against the background of martial law and the catastrophic flooding which took place in Kherson Oblast. Determining the peculiarities and manifestations of epizootic process of rabies in animals was conducted in the conditions of de-occupied territories of Kherson Oblast in 2023. During that year, 27 studies of samples of pathological/biological materials from animals suspected of rabies were conducted. Those included 24 samples from domestic animals – cattle (Bos taurus), dogs (Canis lupus familiaris), cats (Felis silvestris catus), and 3 from wild animals – fox (Vulpes vulpes) and jackals (Canis aureus). According to the results, rabies was confirmed in 88.9% of the total number of analyzed samples. Most often, rabies was diagnosed in domestic animals, particularly, dogs and cats –45.8% and 29.2%, respectively. In the representatives of wild fauna, rabies was detected in 12.5% of the cases, in particular, 8.3% in foxes and 4.2% in jackals. The analysis of the seasonality of rabies morbidity in animals found no pattern in the dynamics. However, according to species, the peak of rabies in foxes took place in autumn, particularly, September and October. An important fact is that the bulk of rabies cases, acccounting for 91.7%, occurred in the period after the Kahovka Dam had been blown up by the Russian Federation. Territorially, during the surveillance period, rabies in animals was confirmed in 23 settlements of Kherson Oblast: 22 cases within the Oblast’s districts and one case in the city of Kherson properPublication In vitro tests of effect of disinfectants on the viability of Heterakis gallinarum nematode eggs during embryogenesis(2024) Євстаф’єва, Валентина Олександрівна; Yevstafieva, V. O.; Омельченко, О. В.; Omelchenko O. V.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Дмитренко, Надія Іванівна; Dmytrenko, N. I.; Крикунова, Валентина Юхимівна; Krykunova, V. E.; Передера, Олена Олександрівна; Peredera, O. O.; Тагільцева, Яніна Михайлівна; Tahiltseva, Y. М.Affecting the transmission of an infection, objects of the external environment become contaminated with pathogens as a result of their massive release by the definitive host during exogenous stages of parasites’ development, which is an important chain of their epizootic process. Therefore, disinfestation plays an important role in the complex of measures for the prevention and control of poultry helminthiases, including Heterakis gallinarum infection of chickens. The purpose of the research was to investigate in laboratory conditions the ovicidal efficiency of modern disinfectants in relation to the viability of developing eggs of H. gallinarum isolated from infested chickens. "Hermecid-VS" (didecyldimethylammonium chloride, glutaraldehyde, benzalkonium chloride) and "Arquadez-plus" (dimethyldialkylammonium chloride, didecyldimethylammonium chloride, tetrasodium salt) disinfectants were tested. These preparations had a high level of ovicidal efficiency against the eggs of Heterakis, "Hermecid-VS" in 0.25% and 0.5% concentration for exposures of 10–60min (93.5–100.0%), "Arquadez-plus" in 1.0% concentration for exposure of 60 min (93.1%), and at 1.5% (96.8–100.0%) and 2.0% (100.0%) concentrations regardless of exposure. The ovicidal effect of disinfectants was characterized by morphological changes in the nematode eggs when using "Hermecid-VS" – accumulation of air bubbles under the shell, thinning and deformation of the shell, death and gradual resorption of the embryo, loosened egg shell and terminated embryonic development, deformation and thinning of egg the shell, and death of the embryo at the stage of formation of the larva; under the effect of "Arquadez-plus", a stop in development at the stage of cleaving blastomeres, destruction of the shell, death and evacuation of the embryo from the egg, shrinkage and gradual defragmentation of the embryo, death of the larva and its defragmentation. Under the action of disinfectants, the metric parameters of Heterakis eggs also changed. Under the influence of "Hermecid-VS" at all concentrations, the length of the eggs and the thickness of the shell were larger (by 2.9–5.4% and 26.7–46.7%), and their width was smaller (by 5.5–6.9%). With the effect of "Arquadez-plus", the length of the eggs was greater (by 0.4–2.0%), and the width was smaller (by 0.7–4.9%) only when using 0.1–2.0% concentrations of the agent. Heterakis egg shell thickness increased under the influence of "Arquadez-plus" in concentrations of 0.25–2.0% (by 33.3–40.0%). The conducted studies allow us to recommend "Hermecid-VS" and "Arquadez-plus" disinfectants in specified concentrations and modes of their use for maintaining epizootic well-being in the complex of measures against Heterakisinfection of chickesnPublication Influence of Ctenocephalides felis on biochemical indicators of blood serum of infested cats(2025) Гаврик, Б. А.; Мельничук, Віталій Васильович; Melnychuk, V. V.; Havryk, B. А.Fleas represent one of the most important groups of blood-sucking parasitic insects. An important aspect of the medical and veterinary importance of fleas, which has not lost its relevance even today, is their role in maintaining natural foci of zooanthroponotic and zoonotic infections. With constant flea attacks on an animal, clinical signs develop, characterized by itching, anxiety, and dermatopathologies, which are due to certain parasite-host relationships, which are based on the interaction of the pathogenic effect of the parasite and the host's defense mechanism. The aim of the research was to determine changes in the biochemical parameters of blood serum of infested cats at different levels of ctenocephalic invasion intensity. The conducted studies have established that flea parasitism on the body of cats led to a negative impact of ectoparasites on the biochemical parameters of the blood serum of infested cats. With increasing intensity of flea infestation of cats, the severity of changes in the biochemical parameters of their blood serum increased. Thus, at low levels of ctenocephalic invasion intensity (up to 19 specimens/head), a decrease in albumin content by 3.99 % (p<0.05) was detected in the blood serum of infected cats compared to the levels in clinically healthy cats. All other indicators were within physiological fluctuations. At higher rates of intensity of ctenocephalic invasion (from 20 to 42 specimens/head) in the blood serum of infected cats, a decrease in albumin content by 15.39 % (p<0.01), glucose by 35.99 % (p<0.05), an increase in total bilirubin content by 33.54 % (p<0.05), urea by 36.24 % (p<0.05), creatinine by 16.18 % (p<0.05), as well as an increase in the activity of enzymes alanine aminotransferase by 22.42 % (p<0.05), aspartate aminotransferase by 38.56 % (p<0.05), alkaline phosphatase by 2.4 times (p<0.05), α-Amylase by 51.44 % (p<0.05), gamma-glutamyl transpeptidase by 31.22 % (p<0.05) compared to the indicators in clinically healthy cats. The results obtained expand existing data on the impact of fleas on the body of infested cats, and also allow us to take into account indicators of infestation intensity in increasing the effectiveness of treatment measuresItem Mollusks (Gastropoda) as Intermediate Hosts of Cattles’ Trematodes (Trematoda) in Conditions of Dnipro Basin’s Small Ponds (Northern Ukraine)(2019-03-28) Фещенко, Д.В.; Бахур, Т.І.; Biricik, H.S.; Антіпов, А.А.; Згозинська, О.А.; Дубова, О.А.; Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Гончаренко, В.П.; Шаганенко, Р.В.; Шаганенко, В.С.; Melnychuk, V. V.; Мельничук, Віталій ВасильовичItem Morphobiological analysis of Trichuris vulpis (Nematoda, Trichuridae), obtained from domestic dogs(2019) Yevstafieva, V. O. ; Євстаф’єва, Валентина Олександрівна; Kravchenko, S. O.; Gutyj, B.; Melnychuk, V. V. ; Мельничук, Віталій Васильович; Kovalenko, P. N.; Volovyk, L. B.; Воловик, Лариса Борисівна; Кравченко, Сергій ОлександровичItem Morphological and Biological Characteristics of Amidostomum anseris (Nematoda, Amidostomatidae) from Anser anser domesticus(2019-03-16) Yevstafieva, V. O.; Євстаф’єва, Валентина Олександрівна ; Стибель, В.В.; Melnychuk, V. V.; Мельничук, Віталій Васильович ; Прийма, О.Б.; Яценко, І.В.; Антіпов, А.А.; Бахур, Т.І.; Гончаренко, В.П.; Підборська, Р.В.; Шаганенко, В.С.; Джміль, В.І.